| Discovery Information |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Truman Chong |
| Year Found | 2023 |
| Location Found | Sawtelle, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.0403 N, 118.44485 W Map |
| Discovery Notes | Moist soil collected in a suburban collection site. Notes, Collected by a tree by a sidewalk by residential housing with heavy foot traffic.
S23-1B:
Instructor: Dr. Tejas Bouklas
Teaching Assistant: Michelle Zorawik
Undergraduate Assistant(s): Clarice Chui, Sophia Sibal Pulido
Team #1 Name: Phushigi
Team #1 Members: Rodia Liu, and Truman Chong
Team #2 Name: Women in Plaque
Team #2 Members: Sasha Anand, Tarissa Almeida, Uyen Trinh, Arieanne De Guzman
Team #3 Name: Plaque Sabbath
Team #3 Members: Khushi Bhatt, Sebastian Castellanos, Edwin Liang, and Alicia Sanoyca |
| Naming Notes | Our group name was Phushigi as in the Fushigi Gravity Ball from the early 2010s. Therefore, it was only fitting for the phage we isolated to be named GravityBall. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 6, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2449 |
| Genome length (bp) | 51100 |
| Character of genome ends | Circularly Permuted |
| GC Content | 60.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AO |
| Subcluster | AO2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques made by this phage are clear and circular with jagged edges. |
| Morphotype | Myoviridae |
| Number of Genes | 78 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PQ999099 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
184 |
| Pitt Freezer Box Grid# |
D10 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
