Arthrobacter phage Timinator
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Detailed Information for Phage Timinator
Discovery Information
Isolation HostArthrobacter sp. ATCC 21022
Found ByTimothy Philip
Year Found2014
Location FoundFrisco, TX USA
Finding InstitutionBaylor University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates33.168693 N, 96.754697 W Map
Discovery NotesThe soil sample was from a construction site in close proximity to heavy duty construction, the road and a Porta-Potty. Furthermore, the soil sample was next to a lot of construction material, like wood, concrete powder and bricks.
Naming NotesEarlier in the process, a lot of plates were completely lysed and the bacteria was completely terminated. Therefore, since my name is Timothy and my phage was terminating the bacteria, I thought Timinator would be a clever play on words.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 20, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage3520
Genome length (bp)51138
Character of genome endsUnknown
GC Content60.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterAO
SubclusterAO2
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesThe plaques changed size over time as it started off as small plaques but during the purification process, the plaques grew in size. The average diameter of the plaques were approximately 3.5 mm.
MorphotypeMyoviridae
Has been Phamerated?Yes
Gene List

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Timinator_1
(HTH DNA binding protein)

Timinator_2
(terminase large subunit)

Timinator_3
(portal protein)

Timinator_4
(capsid maturation protease)

Timinator_5

Timinator_6

Timinator_7
(scaffolding protein)

Timinator_8

Timinator_9
(major capsid protein)

Timinator_10

Timinator_11

Timinator_12

Timinator_13

Timinator_14

Timinator_15
(tail sheath protein)

Timinator_16
(tail tube protein)

Timinator_17

Timinator_18

Timinator_19
(tape measure protein)

Timinator_20
(LysM-like peptidoglycan-binding protein)

Timinator_21
(minor tail protein)

Timinator_22

Timinator_23
(minor tail protein)

Timinator_24
(minor tail protein)

Timinator_25

Timinator_26

Timinator_27
(holin)

Timinator_28
(endolysin)

Timinator_29

Timinator_30
(baseplate wedge protein)

Timinator_31
(baseplate J protein)

Timinator_32
(minor tail protein)

Timinator_33

Timinator_34
(exonuclease)

Timinator_35
(RecT-like ssDNA binding protein)

Timinator_36
(helix-turn-helix DNA binding domain protein)

Timinator_37
(ssDNA-binding protein)

Timinator_38
(helix-turn-helix DNA binding domain protein)

Timinator_39
(helix-turn-helix DNA binding domain protein)

Timinator_40

Timinator_41

Timinator_42
(RusA-like resolvase)

Timinator_43

Timinator_44

Timinator_45

Timinator_46

Timinator_47

Timinator_48

Timinator_49

Timinator_50

Timinator_51

Timinator_52

Timinator_53

Timinator_54

Timinator_55

Timinator_56

Timinator_57

Timinator_58

Timinator_59

Timinator_60

Timinator_61

Timinator_62

Timinator_63

Timinator_64

Timinator_65

Timinator_66

Timinator_67

Timinator_68

Timinator_69

Timinator_70
(DNA binding protein)

Timinator_71

Timinator_72
(acetyltransferase)

Timinator_73
(AlpA-like DNA binding protein)

Timinator_74
(DNA polymerase III sliding clamp beta subunit)

Timinator_75

Timinator_76

Timinator_77

Timinator_78

Timinator_79

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF377441
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 4*10^10 pfu/ml
Date of SEA Lysate Titering Dec 1, 2014
Pitt Freezer Box# 2
Pitt Freezer Box Grid# A6
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload