| Discovery Information |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Taylor Nesbit |
| Year Found | 2014 |
| Location Found | Waco, TX USA |
| Finding Institution | Baylor University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 31.522936 N, 97.155555 W Map |
| Discovery Notes | I discovered this phage from a soil sample taken from a parking lot island littered with trash and other waste in Waco, Texas. |
| Naming Notes | I named this phage LeeroyJ, which is short for "Leeroy Jenkins". The name is a reference to an internet meme and to a viral video posted on YouTube circa 2005. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 23, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 870 |
| Genome length (bp) | 51025 |
| Character of genome ends | Unknown |
| GC Content | 60.9% |
| Sequencing Notes | About 80% of the reads in this sequencing project belonged to an existing phage, Amigo. The other 20% constituted the genome reported here as LeeryoJ. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AO |
| Subcluster | AO2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Baylor University |
| Annotation Status | In GenBank |
| Plaque Notes | The morphology of LeeroyJ varies from having a diameter of .5 millimeters up to 3 millimeters. Initially, after I first isolated the phage, LeeroyJ exhibited a diameter mostly of about 2 to 3 mm. However, after several rounds of purification, the morphology began to take on a diameter mostly ranging from .5 mm to approximately 1.5 mm. |
| Morphotype | Myoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH825703 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2x10^13 pfu/ml |
| Date of SEA Lysate Titering |
Nov 17, 2014 |
| Pitt Freezer Box# |
148 |
| Pitt Freezer Box Grid# |
F5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
