Arthrobacter phage BarretLemon
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Detailed Information for Phage BarretLemon
Discovery Information
Isolation HostArthrobacter sp. ATCC 21022
Found ByEmily Lehmann and Elizabeth Kriese
Year Found2013
Location FoundChippewa Falls, WI USA
Finding InstitutionUniversity of Wisconsin-River Falls
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates44.938522 N, 91.388147 W Map
Discovery NotesThe soil was collected on September 22, 2013 at 3:30 pm in Chippewa Falls WI. It is garden soil composed of horse feces and table scraps located under grass clippings near thriving tomato plants. The soil was very loose and moist. It was a cool 66 degree Fahrenheit sunny fall day.
Naming NotesThe first part of the name is named after Elizabeth's seventh grade biology teacher who inspired her to persue a career in Biology. The seond part of the name is named after Emily's last name. In high school all of her friends would call her Emily Lemon.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 16, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage4324
Genome length (bp)51290
Character of genome endsUnknown
GC Content60.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterAO
SubclusterAO2
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesEmily and Elizabeth took one soil sample and obtained plaques of varying sizes. Elizabeth decided to go forward testing the larger plaques while Emily continued to test the smaller ones. After various comparing various test results we have concluded that it is indeed the same plaque that has varying plaque sizes.
MorphotypeMyoviridae
Number of Genes79
Has been Phamerated?Yes
Gene List

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BarretLemon_1
(HTH DNA binding protein)

BarretLemon_2
(terminase large subunit)

BarretLemon_3
(portal protein)

BarretLemon_4
(capsid maturation protease)

BarretLemon_5

BarretLemon_6

BarretLemon_7
(scaffolding protein)

BarretLemon_8

BarretLemon_9
(major capsid protein)

BarretLemon_10

BarretLemon_11

BarretLemon_12

BarretLemon_13

BarretLemon_14

BarretLemon_15
(tail sheath protein)

BarretLemon_16
(tail tube protein)

BarretLemon_17

BarretLemon_18

BarretLemon_19
(tape measure protein)

BarretLemon_20
(LysM-like peptidoglycan-binding protein)

BarretLemon_21
(minor tail protein)

BarretLemon_22

BarretLemon_23
(minor tail protein)

BarretLemon_24
(minor tail protein)

BarretLemon_25

BarretLemon_26

BarretLemon_27
(holin)

BarretLemon_28
(endolysin)

BarretLemon_29

BarretLemon_30
(baseplate wedge protein)

BarretLemon_31
(baseplate J protein)

BarretLemon_32
(tail protein)

BarretLemon_33

BarretLemon_34
(exonuclease)

BarretLemon_35
(RecT-like ssDNA binding protein)

BarretLemon_36
(helix-turn-helix DNA binding domain protein)

BarretLemon_37
(single-stranded DNA-binding protein)

BarretLemon_38
(helix-turn-helix DNA binding domain protein)

BarretLemon_39
(helix-turn-helix DNA binding domain protein)

BarretLemon_40

BarretLemon_41

BarretLemon_42
(RusA-like resolvase)

BarretLemon_43

BarretLemon_44

BarretLemon_45

BarretLemon_46

BarretLemon_47

BarretLemon_48

BarretLemon_49

BarretLemon_50

BarretLemon_51

BarretLemon_52

BarretLemon_53

BarretLemon_54

BarretLemon_55

BarretLemon_56

BarretLemon_57

BarretLemon_58

BarretLemon_59

BarretLemon_60

BarretLemon_61

BarretLemon_62

BarretLemon_63

BarretLemon_64

BarretLemon_65

BarretLemon_66

BarretLemon_67

BarretLemon_68

BarretLemon_69
(DNA binding protein)

BarretLemon_70

BarretLemon_71
(acetyltransferase)

BarretLemon_72
(AlpA-like DNA binding protein)

BarretLemon_73
(DNA polymerase III sliding clamp beta subunit)

BarretLemon_74

BarretLemon_75

BarretLemon_76

BarretLemon_77

BarretLemon_78

BarretLemon_79

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKU647629
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 4.3X10^9
Date of SEA Lysate Titering Oct 22, 2013
Pitt Freezer Box# 1
Pitt Freezer Box Grid# A7
Available Files
Final DNAMaster FileDownload