Streptomyces phage Karp
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Detailed Information for Phage Karp
Discovery Information
Isolation HostStreptomyces viridochromogenes DSM40736
Found ByIsabel Silverman and Annabelle Laughlin
Year Found2019
Location FoundSt. Louis, MO USA
Finding InstitutionWashington University in St. Louis
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature27°C
GPS Coordinates38.6362 N, 90.2913 W Map
Discovery NotesKarp was found in the St. Louis Zoo is Forest Park. The soil artificially-applied soil (not naturally occuring), layered beneath wood chips
The soil was rich and moist with little organic matter.
Naming NotesWe named our phage after our Washington University Student Associate, Leo Karp.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJun 24, 2020
Sequencing FacilityThe McDonnell Genome Institute at Washington University
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage92
Genome length (bp)130557
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length734 bp
GC Content47.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBK
SubclusterBK1
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionWashington University in St. Louis
Annotation StatusIn GenBank
Plaque NotesSmall plaques, with around a 0.75 mm diameter.
They are not perfectly circular, with jagged but crisp edges.
Number of Genes235
Number of tRNAs34
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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Karp_1

Karp_2

Karp_3

Karp_4

Karp_5

Karp_6

Karp_7
(DNA methyltransferase)

Karp_8
(ThyX-like thymidylate synthase)

Karp_9

Karp_10

Karp_11

Karp_12

Karp_13

Karp_14 — tRNA
(tRNA-Thr(ggt))

Karp_15 — tRNA
(tRNA-Thr(cgt))

Karp_16 — tRNA
(tRNA-Thr(tgt))

Karp_17
(glycosyltransferase)

Karp_18
(glycosyltransferase)

Karp_19
(glycosyltransferase)

Karp_20
(ribonucleotide reductase)

Karp_21
(endolysin)

Karp_22

Karp_23

Karp_24
(portal protein)

Karp_25

Karp_26
(capsid maturation protease)

Karp_27
(major capsid protein)

Karp_28

Karp_29

Karp_30

Karp_31
(head-to-tail adaptor)

Karp_32

Karp_33

Karp_34

Karp_35
(major tail protein)

Karp_36
(tail assembly chaperone)

Karp_37
(tail assembly chaperone)

Karp_38
(tape measure protein)

Karp_39
(minor tail protein)

Karp_40
(minor tail protein)

Karp_41

Karp_42
(minor tail protein)

Karp_43
(minor tail protein)

Karp_44

Karp_45

Karp_46

Karp_47
(HNH endonuclease)

Karp_48
(metallophosphoesterase)

Karp_49

Karp_50
(MerR-like helix-turn-helix DNA binding domain protein)

Karp_51

Karp_52

Karp_53

Karp_54
(DnaB-like dsDNA helicase)

Karp_55

Karp_56
(DNA primase)

Karp_57
(ssDNA binding protein)

Karp_58
(HNH endonuclease)

Karp_59
(DnaE-like DNA polymerase III alpha)

Karp_60

Karp_61
(deoxyuridine triphosphatase)

Karp_62

Karp_63
(RecA-like DNA recombinase)

Karp_64

Karp_65
(PnuC-like nicotinamide riboside transporter)

Karp_66

Karp_67

Karp_68

Karp_69
(Cas4 family exonuclease)

Karp_70

Karp_71
(RuvC-like resolvase)

Karp_72

Karp_73
(DprA-like DNA processing chain A)

Karp_74
(helix-turn-helix DNA binding domain protein)

Karp_75
(glycosyltransferase)

Karp_76
(glycosyltransferase)

Karp_77
(nucleotidyltransferase)

Karp_78

Karp_79
(DnaQ-like DNA polymerase III subunit)

Karp_80
(deoxynucleoside monophosphate kinase)

Karp_81
(5' nucleotidase)

Karp_82

Karp_83

Karp_84

Karp_85

Karp_86
(terminase)

Karp_87

Karp_88

Karp_89 — tRNA
(tRNA-Pro(tgg))

Karp_90 — tRNA
(tRNA-Pro(tgg))

Karp_91
(HNH endonuclease)

Karp_92 — tRNA
(tRNA-Gln(ctg))

Karp_93 — tRNA
(tRNA-Trp(cca))

Karp_94

Karp_95 — tRNA
(tRNA-Ile(gat))

Karp_96 — tRNA
(tRNA-Ser(tga))

Karp_97

Karp_98 — tRNA
(tRNA-Asp(gtc))

Karp_99

Karp_100

Karp_101

Karp_102
(Ro-like RNA binding protein)

Karp_103

Karp_104

Karp_105 — tRNA
(tRNA-Arg(gcg))

Karp_106

Karp_107
(DnaQ-like DNA polymerase III subunit)

Karp_108

Karp_109

Karp_110 — tRNA
(tRNA-Ala(tgc))

Karp_111

Karp_112 — tRNA
(tRNA-Lys(ctt))

Karp_113

Karp_114

Karp_115 — tRNA
(tRNA-Met(cat))

Karp_116 — tRNA
(tRNA-Met(cat))

Karp_117 — tRNA
(tRNA-Phe(gaa))

Karp_118

Karp_119

Karp_120

Karp_121

Karp_122 — tRNA
(tRNA-Tyr(gta))

Karp_123 — tRNA
(tRNA-His(gtg))

Karp_124 — tRNA
(tRNA-Cys(gca))

Karp_125 — tRNA
(tRNA-Asn(gtt))

Karp_126 — tRNA
(tRNA-Arg(tcg))

Karp_127

Karp_128 — tRNA
(tRNA-Arg(cct))

Karp_129 — tRNA
(tRNA-Ser(cga))

Karp_130 — tmRNA
(Tag peptide: ANSKNASAYALAA*)

Karp_131

Karp_132
(band-7-like membrane protein)

Karp_133

Karp_134

Karp_135

Karp_136

Karp_137

Karp_138

Karp_139

Karp_140

Karp_141
(RNA ligase)

Karp_142

Karp_143
(polynucleotide kinase)

Karp_144

Karp_145

Karp_146 — tRNA
(tRNA-Leu(tag))

Karp_147

Karp_148

Karp_149

Karp_150

Karp_151 — tRNA
(tRNA-Val(tac))

Karp_152 — tRNA
(tRNA-Leu(caa))

Karp_153

Karp_154

Karp_155

Karp_156

Karp_157

Karp_158
(NAD-dependent deacetylase)

Karp_159

Karp_160

Karp_161

Karp_162

Karp_163
(metallophosphoesterase)

Karp_164

Karp_165 — tRNA
(tRNA-Lys(ttt))

Karp_166

Karp_167
(WhiB family transcription factor)

Karp_168

Karp_169

Karp_170

Karp_171 — tRNA
(tRNA-Ile(tat))

Karp_172

Karp_173

Karp_174

Karp_175 — tRNA
(tRNA-Glu(ctc))

Karp_176 — tRNA
(tRNA-Glu(ttc))

Karp_177

Karp_178 — tRNA
(tRNA-Gly(gcc))

Karp_179

Karp_180

Karp_181

Karp_182 — tRNA
(tRNA-Gly(tcc))

Karp_183

Karp_184 — tRNA
(tRNA-Gln(ttg))

Karp_185

Karp_186

Karp_187

Karp_188

Karp_189

Karp_190

Karp_191

Karp_192

Karp_193
(thioredoxin)

Karp_194

Karp_195

Karp_196

Karp_197
(nuclease)

Karp_198
(glycosylase)

Karp_199

Karp_200

Karp_201

Karp_202

Karp_203
(RNA ligase)

Karp_204

Karp_205

Karp_206

Karp_207

Karp_208

Karp_209

Karp_210

Karp_211

Karp_212
(Lsr2-like DNA bridging protein)

Karp_213

Karp_214

Karp_215

Karp_216

Karp_217

Karp_218

Karp_219

Karp_220
(HNH endonuclease)

Karp_221

Karp_222

Karp_223

Karp_224

Karp_225

Karp_226
(peptidase)

Karp_227

Karp_228

Karp_229

Karp_230

Karp_231

Karp_232

Karp_233
(HNH endonuclease)

Karp_234
(metallophosphoesterase)

Karp_235

Karp_236
(SprT-like protease)

Karp_237

Karp_238

Karp_239

Karp_240

Karp_241

Karp_242

Karp_243

Karp_244

Karp_245 — tRNA

Karp_246

Karp_247

Karp_248

Karp_249

Karp_250

Karp_251

Karp_252

Karp_253

Karp_254

Karp_255

Karp_256

Karp_257

Karp_258

Karp_259

Karp_260

Karp_261

Karp_262

Karp_263

Karp_264

Karp_265

Karp_266

Karp_267

Karp_268

Karp_269

Karp_270

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMZ820095
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload