Arthrobacter phage Kihatsu
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| Detailed Information for Phage Kihatsu | |
| Discovery Information | |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Reagan Rouland |
| Year Found | 2024 |
| Location Found | Clayton, NC North America |
| Finding Institution | North Carolina State University |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 35.689791 N, 78.424139 W Map |
| Discovery Notes | Utilized the first couple inches of topsoil from a garden bed used to grow vegetables towards the end of the night. Temperatures were cool, around 37°C, and a humidity factor of 72%. |
| Naming Notes | I derived this name from the Japanese words 'Kisei' which means parasitism and 'Bakuhatsu' which means to burst or explode. The derivative combines "ki" and "hatsu" from the words respectively in translation to mean "A parasite that bursts." |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 14, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1823 |
| Genome length (bp) | 43237 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | TCCGCCGCGTGA |
| GC Content | 64.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | FF |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Based on the plagues caused by this phage, it appears to be lytic rather than temperate. |
| Other Cluster Members | |
| Annotating Institution | North Carolina State University |
| Annotation Status | Being Annotated (Expected completion by 5/1/2025) |
| Plaque Notes | Small, cloudy plaques that span a size of roughly 1mm. The phage itself is Siphoviridae with a tail length of ~237.5mm and a head length of ~62.5nm for a total length of ~300nm. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 1x10^10 PFU/mL |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
