| Detailed Information for Phage Kihatsu | |
| Discovery Information | |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Reagan Rouland |
| Year Found | 2024 |
| Location Found | Clayton, NC North America |
| Finding Institution | North Carolina State University |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 35.689791 N, 78.424139 W Map |
| Discovery Notes | Utilized the first couple inches of topsoil from a garden bed used to grow vegetables towards the end of the night. Temperatures were cool, around 37°C, and a humidity factor of 72%. |
| Naming Notes | I derived this name from the Japanese words 'Kisei' which means parasitism and 'Bakuhatsu' which means to burst or explode. The derivative combines "ki" and "hatsu" from the words respectively in translation to mean "A parasite that bursts." |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 14, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 1823 |
| Genome length (bp) | 43237 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | TCCGCCGCGTGA |
| GC Content | 64.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | FF |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Based on the plagues caused by this phage, it appears to be lytic rather than temperate. |
| Other Cluster Members | |
| Annotating Institution | North Carolina State University |
| Annotation Status | In GenBank |
| Plaque Notes | Small, cloudy plaques that span a size of roughly 1mm. The phage itself is Siphoviridae with a tail length of ~237.5mm and a head length of ~62.5nm for a total length of ~300nm. |
| Morphotype | Siphoviridae |
| Number of Genes | 67 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewKihatsu_1 Kihatsu_2 Kihatsu_3 Kihatsu_4 — tRNA Kihatsu_5 Kihatsu_6 Kihatsu_7 Kihatsu_8 Kihatsu_9 Kihatsu_10 Kihatsu_11 Kihatsu_12 Kihatsu_13 Kihatsu_14 Kihatsu_15 Kihatsu_16 Kihatsu_17 Kihatsu_18 Kihatsu_20 Kihatsu_19 Kihatsu_21 Kihatsu_22 Kihatsu_23 Kihatsu_24 Kihatsu_25 Kihatsu_26 Kihatsu_27 Kihatsu_28 Kihatsu_29 Kihatsu_30 Kihatsu_31 Kihatsu_32 Kihatsu_33 Kihatsu_34 Kihatsu_35 Kihatsu_36 Kihatsu_37 Kihatsu_38 Kihatsu_39 — tRNA Kihatsu_40 Kihatsu_41 Kihatsu_42 Kihatsu_43 Kihatsu_44 Kihatsu_45 Kihatsu_46 Kihatsu_47 Kihatsu_48 Kihatsu_49 Kihatsu_50 Kihatsu_51 Kihatsu_52 Kihatsu_53 Kihatsu_54 Kihatsu_55 Kihatsu_56 Kihatsu_57 Kihatsu_58 Kihatsu_59 Kihatsu_60 Kihatsu_61 Kihatsu_62 Kihatsu_63 Kihatsu_64 Kihatsu_65 Kihatsu_66 Kihatsu_67 Kihatsu_68 Kihatsu_69 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PV876942 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1x10^10 PFU/mL |
| Pitt Freezer Box# | 195 |
| Pitt Freezer Box Grid# | C4 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
