Arthrobacter phage Kihatsu
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Detailed Information for Phage Kihatsu
Discovery Information
Isolation HostArthrobacter globiformis B-2979
Found ByReagan Rouland
Year Found2024
Location FoundClayton, NC North America
Finding InstitutionNorth Carolina State University
ProgramPhage Hunters Integrating Research and Education
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates35.689791 N, 78.424139 W Map
Discovery NotesUtilized the first couple inches of topsoil from a garden bed used to grow vegetables towards the end of the night. Temperatures were cool, around 37°C, and a humidity factor of 72%.
Naming NotesI derived this name from the Japanese words 'Kisei' which means parasitism and 'Bakuhatsu' which means to burst or explode. The derivative combines "ki" and "hatsu" from the words respectively in translation to mean "A parasite that bursts."
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 14, 2024
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Sequencer UsedIllumina NextSeq 1000
Read TypeSingle-end reads
Read Length100 bp
Approximate Shotgun Coverage1823
Genome length (bp)43237
Character of genome ends3' Sticky Overhang
Overhang Length12 bases
Overhang SequenceTCCGCCGCGTGA
GC Content64.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterFF
Subcluster--
Cluster Life CycleTemperate
Lysogeny NotesBased on the plagues caused by this phage, it appears to be lytic rather than temperate.
Other Cluster Members
Annotating InstitutionNorth Carolina State University
Annotation StatusIn GenBank
Plaque NotesSmall, cloudy plaques that span a size of roughly 1mm. The phage itself is Siphoviridae with a tail length of ~237.5mm and a head length of ~62.5nm for a total length of ~300nm.
MorphotypeSiphoviridae
Number of Genes67
Number of tRNAs2
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Kihatsu_1

Kihatsu_2
(nucleotidyl transferase)

Kihatsu_3

Kihatsu_4 — tRNA
(tRNA-Ser(gct))

Kihatsu_5
(heilx-turn-helix DNA binding domain)

Kihatsu_6
(terminase, small subunit)

Kihatsu_7
(terminase, large subunit)

Kihatsu_8
(portal protein)

Kihatsu_9
(capsid maturation protease)

Kihatsu_10
(scaffolding protein)

Kihatsu_11
(major capsid protein)

Kihatsu_12

Kihatsu_13
(head-to-tail adaptor)

Kihatsu_14
(head-to-tail stopper)

Kihatsu_15

Kihatsu_16
(tail terminator)

Kihatsu_17
(major tail protein)

Kihatsu_18

Kihatsu_20
(tail assembly chaperone)

Kihatsu_19
(tail assembly chaperone)

Kihatsu_21
(tape measure protein)

Kihatsu_22
(minor tail protein)

Kihatsu_23
(minor tail protein)

Kihatsu_24

Kihatsu_25

Kihatsu_26
(lysin A)

Kihatsu_27

Kihatsu_28
(minor tail protein)

Kihatsu_29
(minor tail protein)

Kihatsu_30

Kihatsu_31

Kihatsu_32

Kihatsu_33
(hydrolase)

Kihatsu_34

Kihatsu_35
(esterase)

Kihatsu_36

Kihatsu_37
(tyrosine integrase)

Kihatsu_38
(lipoprotein)

Kihatsu_39 — tRNA
(tRNA-Arg(tct))

Kihatsu_40
(tyrosine integrase)

Kihatsu_41

Kihatsu_42

Kihatsu_43
(helix-turn-helix DNA binding domain)

Kihatsu_44

Kihatsu_45

Kihatsu_46

Kihatsu_47

Kihatsu_48

Kihatsu_49
(ParB-like N-terminal-like domain)

Kihatsu_50

Kihatsu_51

Kihatsu_52

Kihatsu_53
(helix-turn-helix DNA binding domain)

Kihatsu_54
(DNA polymerase III sliding clamp (Beta))

Kihatsu_55

Kihatsu_56

Kihatsu_57

Kihatsu_58

Kihatsu_59

Kihatsu_60

Kihatsu_61

Kihatsu_62

Kihatsu_63

Kihatsu_64
(HNH endonuclease)

Kihatsu_65
(VRR-Nuc domain protein)

Kihatsu_66

Kihatsu_67

Kihatsu_68

Kihatsu_69
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPV876942
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1x10^10 PFU/mL
Pitt Freezer Box# 195
Pitt Freezer Box Grid# C4
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload