| Detailed Information for Phage LeeroyJ | |
| Discovery Information | |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Taylor Nesbit |
| Year Found | 2014 |
| Location Found | Waco, TX USA |
| Finding Institution | Baylor University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 31.522936 N, 97.155555 W Map |
| Discovery Notes | I discovered this phage from a soil sample taken from a parking lot island littered with trash and other waste in Waco, Texas. |
| Naming Notes | I named this phage LeeroyJ, which is short for "Leeroy Jenkins". The name is a reference to an internet meme and to a viral video posted on YouTube circa 2005. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 23, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 870 |
| Genome length (bp) | 51025 |
| Character of genome ends | Unknown |
| GC Content | 60.9% |
| Sequencing Notes | About 80% of the reads in this sequencing project belonged to an existing phage, Amigo. The other 20% constituted the genome reported here as LeeryoJ. |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AO |
| Subcluster | AO2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewEastWest (None) Beans (AO1) Brent (AO1) Franzy (AO1) Jawnski (AO1) King2 (AO1) NathanVaag (AO1) Piccoletto (AO1) BarretLemon (AO2) BigDome (AO2) BossLady (AO2) GravityBall (AO2) Grekaycon (AO2) JKerns (AO2) Jordan (AO2) LeeroyJ (AO2) Martha (AO2) Pippa (AO2) Shade (AO2) Sicarius2 (AO2) Sonny (AO2) StevieBAY (AO2) TaeYoung (AO2) Timinator (AO2) Wyborn (AO2) Zartrosa (AO2) Abba (AO3) |
| Annotating Institution | Baylor University |
| Annotation Status | In GenBank |
| Plaque Notes | The morphology of LeeroyJ varies from having a diameter of .5 millimeters up to 3 millimeters. Initially, after I first isolated the phage, LeeroyJ exhibited a diameter mostly of about 2 to 3 mm. However, after several rounds of purification, the morphology began to take on a diameter mostly ranging from .5 mm to approximately 1.5 mm. |
| Morphotype | Myoviridae |
| Number of Genes | 79 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewLeeroyJ_1 LeeroyJ_2 LeeroyJ_3 LeeroyJ_4 LeeroyJ_5 LeeroyJ_6 LeeroyJ_7 LeeroyJ_8 LeeroyJ_9 LeeroyJ_10 LeeroyJ_11 LeeroyJ_12 LeeroyJ_13 LeeroyJ_14 LeeroyJ_15 LeeroyJ_16 LeeroyJ_17 LeeroyJ_18 LeeroyJ_19 LeeroyJ_20 LeeroyJ_21 LeeroyJ_22 LeeroyJ_23 LeeroyJ_24 LeeroyJ_25 LeeroyJ_26 LeeroyJ_27 LeeroyJ_28 LeeroyJ_29 LeeroyJ_30 LeeroyJ_31 LeeroyJ_32 LeeroyJ_33 LeeroyJ_34 LeeroyJ_35 LeeroyJ_36 LeeroyJ_37 LeeroyJ_38 LeeroyJ_39 LeeroyJ_40 LeeroyJ_41 LeeroyJ_42 LeeroyJ_43 LeeroyJ_44 LeeroyJ_45 LeeroyJ_46 LeeroyJ_47 LeeroyJ_48 LeeroyJ_49 LeeroyJ_50 LeeroyJ_51 LeeroyJ_52 LeeroyJ_53 LeeroyJ_54 LeeroyJ_55 LeeroyJ_56 LeeroyJ_57 LeeroyJ_58 LeeroyJ_59 LeeroyJ_60 LeeroyJ_61 LeeroyJ_62 LeeroyJ_63 LeeroyJ_64 LeeroyJ_65 LeeroyJ_66 LeeroyJ_67 LeeroyJ_68 LeeroyJ_69 LeeroyJ_70 LeeroyJ_71 LeeroyJ_72 LeeroyJ_73 LeeroyJ_74 LeeroyJ_75 LeeroyJ_76 LeeroyJ_77 LeeroyJ_78 LeeroyJ_79 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH825703 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 2x10^13 pfu/ml |
| Date of SEA Lysate Titering | Nov 17, 2014 |
| Pitt Freezer Box# | 148 |
| Pitt Freezer Box Grid# | F5 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
