Gordonia phage MakoManhole
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Detailed Information for Phage MakoManhole
Discovery Information
Isolation HostGordonia rubripertincta NRRL B-16540
Found ByChristopher Huang
Year Found2023
Location FoundDavie, FL United States
Finding InstitutionNova Southeastern University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates26.07439 N, 80.24096 W Map
Discovery NotesThe soil sample was collected at a sunny day at 85 degrees Fahrenheit. It rained two days prior. The soil was collected between a road and a small river near a manhole cover. The soil collected contained some vegetation.
Naming NotesMako is the name of a building on campus, and the phage was collected near a manhole.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 2, 2024
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage644
Genome length (bp)61592
Character of genome endsCircularly Permuted
GC Content68.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterDR
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionNova Southeastern University
Annotation StatusIn GenBank
Plaque NotesThe plaques were relatively clear. Some of them seemed to vary in size.
Number of Genes83
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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MakoManhole_1
(ParB-like nuclease domain protein)

MakoManhole_2
(oxidoreductase)

MakoManhole_3
(aGPT-Pplase2 domain protein)

MakoManhole_4
(adenylate kinase)

MakoManhole_5

MakoManhole_6
(terminase)

MakoManhole_7

MakoManhole_8
(RuvC-like resolvase)

MakoManhole_9

MakoManhole_10
(portal protein)

MakoManhole_11
(capsid maturation protease)

MakoManhole_12

MakoManhole_13
(major capsid hexamer protein)

MakoManhole_14
(major capsid pentamer protein)

MakoManhole_15

MakoManhole_16

MakoManhole_17

MakoManhole_18

MakoManhole_19

MakoManhole_20
(major tail protein)

MakoManhole_21
(head-to-tail adaptor)

MakoManhole_22

MakoManhole_23

MakoManhole_24

MakoManhole_25
(tail assembly chaperone)

MakoManhole_26
(tape measure protein)

MakoManhole_27
(minor tail protein)

MakoManhole_28
(minor tail protein)

MakoManhole_29

MakoManhole_30

MakoManhole_31

MakoManhole_32

MakoManhole_33

MakoManhole_34

MakoManhole_35

MakoManhole_36

MakoManhole_37

MakoManhole_38

MakoManhole_39

MakoManhole_40
(DNA binding protein)

MakoManhole_41

MakoManhole_42

MakoManhole_43
(lysin A, L-Ala-D-Glu peptidase domain)

MakoManhole_44
(lysin A, glycosyl hydrolase domain)

MakoManhole_45
(lysin B)

MakoManhole_46

MakoManhole_47
(exonuclease)

MakoManhole_48
(DNA helicase)

MakoManhole_49
(RepA-like helicase)

MakoManhole_50
(HNH endonuclease)

MakoManhole_51
(DNA polymerase I)

MakoManhole_52

MakoManhole_53

MakoManhole_54

MakoManhole_55

MakoManhole_56

MakoManhole_57

MakoManhole_58

MakoManhole_59
(ribbon-helix-helix DNA binding domain protein)

MakoManhole_60

MakoManhole_61

MakoManhole_62
(helix-turn-helix DNA binding domain)

MakoManhole_63

MakoManhole_64

MakoManhole_65

MakoManhole_66

MakoManhole_67

MakoManhole_68

MakoManhole_69

MakoManhole_70
(DNA binding protein)

MakoManhole_71

MakoManhole_72

MakoManhole_73

MakoManhole_74
(HNH endonuclease)

MakoManhole_75
(HNH endonuclease)

MakoManhole_76

MakoManhole_77
(DNA binding protein)

MakoManhole_78

MakoManhole_79

MakoManhole_80

MakoManhole_81

MakoManhole_82

MakoManhole_83

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPQ184807
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 184
Pitt Freezer Box Grid# G6
Available Files
GenBank File for PhameratorDownload