| Detailed Information for Phage Manuel | |
| Discovery Information | |
| Isolation Host | Streptomyces lividans JI 1326 |
| Found By | Christian Laing |
| Year Found | 2013 |
| Location Found | Wilkes-Barre, PA USA |
| Finding Institution | Wilkes University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 41.244149 N, 75.88977 W Map |
| Discovery Notes | Collected from soil sample by the entrance of the parking by the Henry Student Center at Wilkes University. Phage obtained from direct plating. |
| Naming Notes | Named after my father |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 17, 2014 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 |
| Sequencer Used | 454 Genome Sequencer FLX |
| Approximate Shotgun Coverage | 100 |
| Genome length (bp) | 45177 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 276 bp |
| End Determination Notes | Buildups of reads in 454 data flanking a region of double coverage. |
| GC Content | 60.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BF |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Morphotype | Podoviridae |
| Number of Genes | 63 |
| Number of tRNAs | 20 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewManuel_1 Manuel_2 Manuel_3 Manuel_4 Manuel_5 Manuel_6 Manuel_7 Manuel_8 Manuel_9 Manuel_10 Manuel_11 Manuel_12 Manuel_13 Manuel_14 Manuel_15 — tRNA Manuel_16 — tRNA Manuel_17 — tRNA Manuel_18 — tRNA Manuel_19 — tRNA Manuel_20 — tRNA Manuel_21 — tRNA Manuel_22 — tRNA Manuel_23 — tRNA Manuel_24 — tRNA Manuel_25 — tRNA Manuel_26 — tRNA Manuel_27 — tRNA Manuel_28 — tRNA Manuel_29 — tRNA Manuel_30 — tRNA Manuel_31 — tRNA Manuel_32 — tRNA Manuel_33 — tRNA Manuel_34 Manuel_35 Manuel_36 Manuel_37 Manuel_38 Manuel_39 Manuel_40 Manuel_41 Manuel_42 Manuel_43 Manuel_44 Manuel_45 Manuel_46 Manuel_47 Manuel_48 Manuel_49 Manuel_50 Manuel_51 Manuel_52 Manuel_53 Manuel_54 Manuel_55 Manuel_56 Manuel_57 Manuel_58 Manuel_59 Manuel_60 Manuel_61 Manuel_62 Manuel_63 — tRNA Manuel_64 Manuel_65 Manuel_66 Manuel_67 Manuel_68 Manuel_69 Manuel_70 Manuel_71 Manuel_72 Manuel_73 Manuel_74 Manuel_75 Manuel_76 Manuel_77 Manuel_78 Manuel_79 Manuel_80 Manuel_81 Manuel_82 Manuel_83 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG518519 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.1x10^9 |
| Date of SEA Lysate Titering | Nov 18, 2013 |
| Pitt Freezer Box# | 124 |
| Pitt Freezer Box Grid# | E3 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
