Streptomyces phage MeganTheeKilla
MeganTheeKilla Plaque Picture
MeganTheeKilla EM Picture
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(S. griseus NBRC13350)
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(S. scabiei 82-22)
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Detailed Information for Phage MeganTheeKilla
Discovery Information
Isolation HostStreptomyces viridochromogenes DSM40736
Found ByJennifer Olabode, Khushi Talati
Year Found2019
Location FoundRichmond, TX United States
Finding InstitutionWashington University in St. Louis
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature88°C
GPS Coordinates29.666 N, 95.731 W Map
Discovery NotesThe soil is hard, dry, and crumbly. The depth of the hole is 6 cm. The soil was from a patch of dying grass in the backyard of Jennifer's house.
Naming NotesMeganTheeStallion is an up-and-coming rapper from Houston. Because the sample was found in Houston, we dedicated our phage to this artist.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJun 15, 2020
Sequencing FacilityThe McDonnell Genome Institute at Washington University
Shotgun Sequencing MethodIllumina
Sequencer UsedIllumina MiSeq
Read TypeSingle-end reads
Read Length150 bp
Approximate Shotgun Coverage93
Genome length (bp)128152
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length766 bp
GC Content47.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBK
SubclusterBK1
Cluster Life CycleLytic
Other Cluster Members

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Annadreamy (BK1)

Belfort (BK1)

Beuffert (BK1)

Blueeyedbeauty (BK1)

Comrade (BK1)

Emma1919 (BK1)

Faust (BK1)

Forrest (BK1)

Francob (BK1)

Gilson (BK1)

Jada (BK1)

Karp (BK1)

Kenrey (BK1)

Limpid (BK1)

MeganTheeKilla (BK1)

Moab (BK1)

Patelgo (BK1)

Phredrick (BK1)

SeresaTree (BK1)

Sham (BK1)

SparkleGoddess (BK1)

Stigma (BK1)

TunaTartare (BK1)

Westy (BK1)

BillNye (BK2)

Circinus (BK2)

Muntaha (BK2)

Wakanda (BK2)
Annotating InstitutionWashington University in St. Louis
Annotation StatusIn GenBank
Plaque NotesThe plaques formed on our plates were consistently sized, often between 0.5 and 1.5 mm in diameter, with clean and distinct edges. Most plaques were circular in shape, if not perfectly round.
MorphotypeSiphoviridae
Number of Genes233
Number of tRNAs36
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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MeganTheeKilla_1

MeganTheeKilla_2

MeganTheeKilla_3

MeganTheeKilla_4

MeganTheeKilla_5

MeganTheeKilla_6

MeganTheeKilla_7
(DNA methyltransferase)

MeganTheeKilla_8
(ThyX-like thymidylate synthase)

MeganTheeKilla_9

MeganTheeKilla_10

MeganTheeKilla_11

MeganTheeKilla_12

MeganTheeKilla_13

MeganTheeKilla_14 — tRNA
(tRNA-Thr(ggt))

MeganTheeKilla_15 — tRNA
(tRNA-Thr(cgt))

MeganTheeKilla_16 — tRNA
(tRNA-Thr(tgt))

MeganTheeKilla_17 — tRNA
(tRNA-Ile(gat))

MeganTheeKilla_18

MeganTheeKilla_19
(hydrolase)

MeganTheeKilla_20
(glycosyltransferase)

MeganTheeKilla_21
(glycosyltransferase)

MeganTheeKilla_22
(ribonucleotide reductase)

MeganTheeKilla_23
(endolysin)

MeganTheeKilla_24

MeganTheeKilla_25

MeganTheeKilla_26
(portal protein)

MeganTheeKilla_27

MeganTheeKilla_28
(capsid maturation protease)

MeganTheeKilla_29
(major capsid protein)

MeganTheeKilla_30

MeganTheeKilla_31
(head-to-tail adaptor)

MeganTheeKilla_32
(head-to-tail stopper)

MeganTheeKilla_33

MeganTheeKilla_34

MeganTheeKilla_35
(major tail protein)

MeganTheeKilla_36
(tail assembly chaperone)

MeganTheeKilla_37
(tail assembly chaperone)

MeganTheeKilla_38
(tape measure protein)

MeganTheeKilla_39
(minor tail protein)

MeganTheeKilla_40
(minor tail protein)

MeganTheeKilla_41

MeganTheeKilla_42
(minor tail protein)

MeganTheeKilla_43
(minor tail protein)

MeganTheeKilla_44

MeganTheeKilla_45

MeganTheeKilla_46

MeganTheeKilla_47
(HNH endonuclease)

MeganTheeKilla_48
(metallophosphoesterase)

MeganTheeKilla_49

MeganTheeKilla_50
(MerR-like helix-turn-helix DNA binding domain protein)

MeganTheeKilla_51

MeganTheeKilla_52

MeganTheeKilla_53

MeganTheeKilla_54
(DnaB-like dsDNA helicase)

MeganTheeKilla_55

MeganTheeKilla_56
(DNA primase)

MeganTheeKilla_57
(ssDNA binding protein)

MeganTheeKilla_58
(HNH endonuclease)

MeganTheeKilla_59
(DnaE-like DNA polymerase III alpha)

MeganTheeKilla_60

MeganTheeKilla_61
(MazG-like nucleotide pyrophosphohydrolase)

MeganTheeKilla_62

MeganTheeKilla_63
(RecA-like DNA recombinase)

MeganTheeKilla_64

MeganTheeKilla_65

MeganTheeKilla_66
(PnuC-like nicotinamide riboside transporter)

MeganTheeKilla_67
(Holliday junction resolvase)

MeganTheeKilla_68

MeganTheeKilla_69

MeganTheeKilla_70
(Cas4 family exonuclease)

MeganTheeKilla_71

MeganTheeKilla_72
(RuvC-like resolvase)

MeganTheeKilla_73

MeganTheeKilla_74
(DprA-like ssDNA binding protein)

MeganTheeKilla_75
(helix-turn-helix DNA-binding domain protein)

MeganTheeKilla_76
(glycosyltransferase)

MeganTheeKilla_77
(glycosyltransferase)

MeganTheeKilla_78
(nucleotidyltransferase)

MeganTheeKilla_79

MeganTheeKilla_80
(DnaQ-like DNA polymerase III subunit)

MeganTheeKilla_81
(deoxynucleoside monophosphate kinase)

MeganTheeKilla_82
(5' nucleotidase)

MeganTheeKilla_83

MeganTheeKilla_84

MeganTheeKilla_85

MeganTheeKilla_86

MeganTheeKilla_87
(terminase large subunit)

MeganTheeKilla_88

MeganTheeKilla_89

MeganTheeKilla_90 — tRNA
(tRNA-Pro(tgg))

MeganTheeKilla_91 — tRNA
(tRNA-Pro(tgg))

MeganTheeKilla_92
(HNH endonuclease)

MeganTheeKilla_93

MeganTheeKilla_94 — tRNA
(tRNA-Gln(ctg))

MeganTheeKilla_95 — tRNA
(tRNA-Trp(cca))

MeganTheeKilla_96

MeganTheeKilla_97 — tRNA
(tRNA-Ile(gat))

MeganTheeKilla_98 — tRNA
(tRNA-Ser(tga))

MeganTheeKilla_99

MeganTheeKilla_100 — tRNA
(tRNA-Asp(gtc))

MeganTheeKilla_101

MeganTheeKilla_102

MeganTheeKilla_103

MeganTheeKilla_104
(Ro-like RNA binding protein)

MeganTheeKilla_105

MeganTheeKilla_106

MeganTheeKilla_107

MeganTheeKilla_108

MeganTheeKilla_109 — tRNA
(tRNA-Arg(gcg))

MeganTheeKilla_110

MeganTheeKilla_111
(DnaQ-like DNA polymerase III subunit)

MeganTheeKilla_112

MeganTheeKilla_113

MeganTheeKilla_114 — tRNA
(tRNA-Ala(tgc))

MeganTheeKilla_115

MeganTheeKilla_116 — tRNA
(tRNA-Lys(ctt))

MeganTheeKilla_117

MeganTheeKilla_118 — tRNA
(tRNA-Met(cat))

MeganTheeKilla_119 — tRNA
(tRNA-Met(cat))

MeganTheeKilla_120 — tRNA
(tRNA-Phe(gaa))

MeganTheeKilla_121

MeganTheeKilla_122

MeganTheeKilla_123

MeganTheeKilla_124

MeganTheeKilla_125 — tRNA
(tRNA-Tyr(gta))

MeganTheeKilla_126 — tRNA
(tRNA-His(gtg))

MeganTheeKilla_127 — tRNA
(tRNA-Cys(gca))

MeganTheeKilla_128 — tRNA
(tRNA-Asn(gtt))

MeganTheeKilla_129 — tRNA
(tRNA-Arg(tcg))

MeganTheeKilla_130

MeganTheeKilla_131

MeganTheeKilla_132 — tRNA
(tRNA-Arg(cct))

MeganTheeKilla_133

MeganTheeKilla_134 — tmRNA
(Tag peptide: ANSNFALAA*)

MeganTheeKilla_135

MeganTheeKilla_136
(band-7-like membrane protein)

MeganTheeKilla_137

MeganTheeKilla_138

MeganTheeKilla_139

MeganTheeKilla_140

MeganTheeKilla_141 — tRNA
(tRNA-Leu(gag))

MeganTheeKilla_142

MeganTheeKilla_143

MeganTheeKilla_144

MeganTheeKilla_145 — tRNA
(tRNA-Ser(gct))

MeganTheeKilla_146

MeganTheeKilla_147
(RNA ligase)

MeganTheeKilla_148

MeganTheeKilla_149

MeganTheeKilla_150
(polynucleotide kinase)

MeganTheeKilla_151

MeganTheeKilla_152

MeganTheeKilla_153
(HNH endonuclease)

MeganTheeKilla_154 — tRNA
(tRNA-Leu(tag))

MeganTheeKilla_155

MeganTheeKilla_156

MeganTheeKilla_157

MeganTheeKilla_158

MeganTheeKilla_159 — tRNA
(tRNA-Val(tac))

MeganTheeKilla_160 — tRNA
(tRNA-Leu(caa))

MeganTheeKilla_161

MeganTheeKilla_162

MeganTheeKilla_163

MeganTheeKilla_164

MeganTheeKilla_165

MeganTheeKilla_166

MeganTheeKilla_167

MeganTheeKilla_168

MeganTheeKilla_169

MeganTheeKilla_170
(rIIB-like protein)

MeganTheeKilla_171

MeganTheeKilla_172
(metallophosphoesterase)

MeganTheeKilla_173

MeganTheeKilla_174

MeganTheeKilla_175 — tRNA
(tRNA-Lys(ttt))

MeganTheeKilla_176

MeganTheeKilla_177
(WhiB family transcription factor)

MeganTheeKilla_178

MeganTheeKilla_179

MeganTheeKilla_180

MeganTheeKilla_181 — tRNA
(tRNA-Ile(tat))

MeganTheeKilla_182

MeganTheeKilla_183

MeganTheeKilla_184

MeganTheeKilla_185 — tRNA
(tRNA-Glu(ctc))

MeganTheeKilla_186 — tRNA
(tRNA-Glu(ttc))

MeganTheeKilla_187

MeganTheeKilla_188 — tRNA
(tRNA-Gly(gcc))

MeganTheeKilla_189

MeganTheeKilla_190 — tRNA
(tRNA-Gly(tcc))

MeganTheeKilla_191

MeganTheeKilla_192 — tRNA
(tRNA-Gln(ttg))

MeganTheeKilla_193

MeganTheeKilla_194

MeganTheeKilla_195

MeganTheeKilla_196

MeganTheeKilla_197

MeganTheeKilla_198

MeganTheeKilla_199

MeganTheeKilla_200

MeganTheeKilla_201
(thioredoxin)

MeganTheeKilla_202

MeganTheeKilla_203

MeganTheeKilla_204

MeganTheeKilla_205

MeganTheeKilla_206

MeganTheeKilla_207

MeganTheeKilla_208

MeganTheeKilla_209

MeganTheeKilla_210
(RNA ligase)

MeganTheeKilla_211

MeganTheeKilla_212

MeganTheeKilla_213

MeganTheeKilla_214

MeganTheeKilla_215

MeganTheeKilla_216

MeganTheeKilla_217

MeganTheeKilla_218

MeganTheeKilla_219

MeganTheeKilla_220

MeganTheeKilla_221
(Lsr2-like DNA bridging protein)

MeganTheeKilla_222

MeganTheeKilla_223

MeganTheeKilla_224

MeganTheeKilla_225

MeganTheeKilla_226

MeganTheeKilla_227

MeganTheeKilla_228

MeganTheeKilla_229
(HNH endonuclease)

MeganTheeKilla_230

MeganTheeKilla_231

MeganTheeKilla_232

MeganTheeKilla_233
(peptidase)

MeganTheeKilla_234

MeganTheeKilla_235

MeganTheeKilla_236

MeganTheeKilla_237

MeganTheeKilla_238

MeganTheeKilla_239

MeganTheeKilla_240

MeganTheeKilla_241
(metallophosphoesterase)

MeganTheeKilla_242

MeganTheeKilla_243
(SprT-like protease)

MeganTheeKilla_244

MeganTheeKilla_245

MeganTheeKilla_246

MeganTheeKilla_247

MeganTheeKilla_248

MeganTheeKilla_249

MeganTheeKilla_250 — tRNA
(tRNA-?(Asn|Lys)(tt))

MeganTheeKilla_251

MeganTheeKilla_252

MeganTheeKilla_253
(kinase)

MeganTheeKilla_254

MeganTheeKilla_255

MeganTheeKilla_256

MeganTheeKilla_257

MeganTheeKilla_258

MeganTheeKilla_259

MeganTheeKilla_260

MeganTheeKilla_261

MeganTheeKilla_262

MeganTheeKilla_263

MeganTheeKilla_264

MeganTheeKilla_265

MeganTheeKilla_266

MeganTheeKilla_267

MeganTheeKilla_268

MeganTheeKilla_269

MeganTheeKilla_270
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMW435853
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload