| Detailed Information for Phage Nightmare |
| Discovery Information |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Morgan Sperratore |
| Year Found | 2015 |
| Location Found | Chester, PA USA |
| Finding Institution | Cabrini University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 23°C |
| GPS Coordinates | 39.86284 N, 75.371462 W Map |
| Discovery Notes | Soil was dry and in a shady spot. |
| Naming Notes | Soil sample was taken from my grandparent's house and my grandmother had a nightmare. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 26, 2016 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 160 |
| Genome length (bp) | 58839 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGGCCT |
| End Determination Notes | CGCCGGCCT |
| GC Content | 49.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AU |
| Subcluster | AU1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Nightmare has medium size, clear plaques. |
| Morphotype | Siphoviridae |
| Number of Genes | 92 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF140423 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
Cabrini College |
| SEA Lysate Titer |
7.3 X 10^9 pfu/ml |
| Date of SEA Lysate Titering |
Aug 13, 2015 |
| Pitt Freezer Box# |
149 |
| Pitt Freezer Box Grid# |
C12 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
