| Detailed Information for Phage Prager |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jackelyn Prager |
| Year Found | 2023 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.444638 N, 79.952252 W Map |
| Discovery Notes | This phage was isolated from a soil sample collected by Jackie Prager on 11/8/2023. Phage isolation and purification were performed by Deborah Jacobs-Sera. Jackie, a producer at CNN, was here to document phage hunting for a CNN International piece. |
| Naming Notes | Jackie named it after herself! |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 7, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Paired-end reads |
| Read Length | 300 bp |
| Approximate Shotgun Coverage | 10485 |
| Genome length (bp) | 64462 |
| Character of genome ends | Circularly Permuted |
| GC Content | 59.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | D |
| Subcluster | D1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | The plaues are tiny, ~2mm, and realtively clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 88 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PQ868991 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
219 |
| Pitt Freezer Box Grid# |
F1 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
