Mycobacterium phage Prager
Prager EM Picture
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Detailed Information for Phage Prager
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJackelyn Prager
Year Found2023
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramPhage Hunters Integrating Research and Education
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates40.444638 N, 79.952252 W Map
Discovery NotesThis phage was isolated from a soil sample collected by Jackie Prager on 11/8/2023. Phage isolation and purification were performed by Deborah Jacobs-Sera. Jackie, a producer at CNN, was here to document phage hunting for a CNN International piece.
Naming NotesJackie named it after herself!
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 7, 2024
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage10485
Genome length (bp)64462
Character of genome endsCircularly Permuted
GC Content59.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterD
SubclusterD1
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesThe plaues are tiny, ~2mm, and realtively clear.
MorphotypeSiphoviridae
Number of Genes88
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Prager_1

Prager_2

Prager_3

Prager_4
(capsid accessory protein)

Prager_5

Prager_6

Prager_7
(VIP2-like toxin)

Prager_8
(terminase)

Prager_9
(portal protein)

Prager_10

Prager_11
(capsid maturation protease)

Prager_12

Prager_13

Prager_14
(scaffolding protein)

Prager_15
(minor capsid protein)

Prager_16
(major capsid protein)

Prager_17

Prager_18

Prager_19
(head-to-tail stopper)

Prager_20

Prager_21
(tail terminator)

Prager_22

Prager_23

Prager_24
(major tail protein)

Prager_25

Prager_26

Prager_27
(tape measure protein)

Prager_28
(minor tail protein)

Prager_29
(minor tail protein)

Prager_30
(minor tail protein)

Prager_31
(minor tail protein)

Prager_32
(minor tail protein)

Prager_33

Prager_34

Prager_35
(lysin A)

Prager_36

Prager_37

Prager_38

Prager_39

Prager_40
(lysin B)

Prager_41

Prager_42

Prager_43

Prager_44

Prager_45

Prager_46

Prager_47

Prager_48

Prager_49

Prager_50

Prager_51

Prager_52

Prager_53
(DNA helicase)

Prager_54

Prager_55

Prager_56

Prager_57
(exonuclease)

Prager_58

Prager_59
(ASCE ATPase)

Prager_60

Prager_61

Prager_62
(oxidoreductase)

Prager_63
(5' nucleotidase)

Prager_64
(methyltransferase)

Prager_65

Prager_66
(FabG-like reductase)

Prager_67
(DnaE-like DNA polymerase III)

Prager_68

Prager_69
(ThyX-like thymidylate synthase)

Prager_70

Prager_71
(RuvC-like resolvase)

Prager_72

Prager_73

Prager_74

Prager_75
(helix-turn-helix DNA-binding protein)

Prager_76

Prager_77

Prager_78

Prager_79

Prager_80

Prager_81

Prager_82

Prager_83

Prager_84

Prager_85

Prager_86

Prager_87

Prager_88
(DNA primase/polymerase)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPQ868991
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload