| Detailed Information for Phage RetrieverFever |
| Discovery Information |
| Isolation Host | Streptomyces scabiei RL-34 |
| Found By | Quynh Pham |
| Year Found | 2020 |
| Location Found | Clarksburg, MD United States |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.23 N, 77.24 W Map |
| Discovery Notes | Collected 8/10/20, 9:35 AM, 80 °F, 75% humidity Soil under grass and brush under Eastern red cedar tree, ~2.25" deep. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 14, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2778 |
| Genome length (bp) | 46175 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 253 bp |
| GC Content | 60.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BF |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Maryland, Baltimore County |
| Annotation Status | In GenBank |
| Morphotype | Podoviridae |
| Number of Genes | 66 |
| Number of tRNAs | 21 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MZ622160 |
| Refseq Number | None yet |
| Published in Paper | Davis et al 2022, Microbiology Resource Announcements: Genome Sequences of Streptomyces Bacteriophages Fabian, FlowerPower, Geostin, RetrieverFever, and Vorvolakos |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.62e9 pfu/mL |
| Pitt Freezer Box# |
117 |
| Pitt Freezer Box Grid# |
A6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
