| Detailed Information for Phage Romero | |
| Discovery Information | |
| Isolation Host | Streptomyces griseus ATCC 10137 |
| Found By | Zandra Romero |
| Year Found | 2017 |
| Location Found | Venus, TX USA |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 25°C |
| GPS Coordinates | 32.416202 N, 97.100147 W Map |
| Discovery Notes | I collected the soil sample on January 15, 2017. The weather was cloudy and it rained the day before. The soil can be described as muddy, wet, it had plant roots, leaves. |
| Naming Notes | I decided to name it after my family last name. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 2, 2017 |
| Sequencing Facility | University of North Texas |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 209 |
| Genome length (bp) | 46079 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 264 bp |
| GC Content | 59.7% |
| Sequencing Notes | While one end of the terminal repeat was obvious in the sequencing reads for this project, the other end was far less so. A buildup of reads was observed at a position that matched the right side of the terminal repeat in other similar phages, so that was selected as the right end of the terminal repeat. |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BF |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Average diameter of plaques is around 1.5 mm. Plaques were normally clear and round. |
| Morphotype | Podoviridae |
| Number of Genes | 64 |
| Number of tRNAs | 22 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewRomero_1 Romero_2 Romero_3 Romero_4 Romero_5 Romero_6 Romero_7 Romero_8 Romero_9 Romero_10 Romero_11 Romero_12 Romero_13 Romero_14 — tRNA Romero_15 — tRNA Romero_16 — tRNA Romero_17 — tRNA Romero_18 — tRNA Romero_19 — tRNA Romero_20 — tRNA Romero_21 — tRNA Romero_22 — tRNA Romero_23 — tRNA Romero_24 — tRNA Romero_25 — tRNA Romero_26 — tRNA Romero_27 — tRNA Romero_28 — tRNA Romero_29 — tRNA Romero_30 — tRNA Romero_31 — tRNA Romero_32 — tRNA Romero_33 — tRNA Romero_34 — tRNA Romero_35 — tRNA Romero_36 Romero_37 Romero_38 Romero_39 Romero_40 Romero_41 Romero_42 Romero_43 Romero_44 Romero_45 Romero_46 Romero_47 Romero_48 Romero_49 Romero_50 Romero_51 Romero_52 Romero_53 Romero_54 Romero_55 Romero_56 Romero_57 Romero_58 Romero_59 Romero_60 Romero_61 Romero_62 Romero_63 Romero_64 Romero_65 Romero_66 Romero_67 Romero_68 Romero_69 Romero_70 Romero_71 Romero_72 Romero_73 Romero_74 Romero_75 Romero_76 Romero_77 Romero_78 Romero_79 Romero_80 Romero_81 Romero_82 Romero_83 Romero_84 Romero_85 Romero_86 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG663584 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 70 |
| Pitt Freezer Box Grid# | D6 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
