| Detailed Information for Phage SchoolBus |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Team Afplaq |
| Year Found | 2017 |
| Location Found | Santa Monica, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 35°C |
| GPS Coordinates | 34.031111 N, 118.473333 W Map |
| Discovery Notes | Phage SchoolBus was isolated from soil, collected on 10/07/17 by Kyle Scranton,
at a depth of about 2 inches from soil taken under a park bench near a trash can. This sample was mostly dry soil w/ small pieces of wood. Air temp at time of collection was 26.7˚C.
SchoolBus was isolated by direct plating at 35C.
Characterization was performed by Team Afplaq (Jason Eigenbrood, Nima Hooshdaran, Kyle Scranton).
Phage morphology under EM shows siphoviridae with prolate head characteristic of cluster O phages.
Midi-Lysate (Archive) Titer: 1.6 x 1014
Date of High-titer Lysate Titering: 11/08/17
MIMG103AL-F17 Instructor: Dr. Amanda Freise
Teaching Assistant: Andrew Lund
Undergraduate Assistant: Keith Mitchell
Laboratory Support: Dr. Krisanavane Reddi and Dr. Jordan Moberg Parker |
| Naming Notes | We decided to name our phage SchoolBus because it looked like a school bus to our team. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 21, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1254 |
| Genome length (bp) | 71651 |
| Character of genome ends | Unknown |
| Overhang Length | 4 bases |
| Overhang Sequence | GTGT |
| GC Content | 65.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | O |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | In GenBank |
| Plaque Notes | Clear
Average size (and size range) diameter in mm: Average=1.0mm; Range=0.75mm-1.25mm |
| Morphotype | Siphoviridae |
| Number of Genes | 128 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH020246 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
58 |
| Pitt Freezer Box Grid# |
F7 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
