| Detailed Information for Phage Schuy | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Alexander DiGiacomo |
| Year Found | 2023 |
| Location Found | Conshohoken, PA United States |
| Finding Institution | Saint Joseph's University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.06936 N, 75.30401 W Map |
| Discovery Notes | The soil was collected on 1/12/23 at 1:10 pm. The sample was collected on the bank of the Schuylkill River. The collection site was just beyond the parking lot of the residence Inn by Marriot (191 Washington St, Conshohocken, PA 19428). Description of the site: enter walking path alng the parking lot, travel down the bank 3 feet from path and 10 feet from river. Soil collected on the bank slope about 2 feet from base of tree. The soil was dry, and sandy. The weather had been consistently brisk, dry, and cloudy before the date of extraction. The soil was extracted 28.2 cm down. |
| Naming Notes | The soil sample Schuy was isolated from was taken from the bank of the Schuylkill River-- a notable Pennsylvanian landmark. The phage was named after the river, shortening the name to the nickname of "Schuy". |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 19, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1317 |
| Genome length (bp) | 70853 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 4 bases |
| Overhang Sequence | GTCT |
| GC Content | 65.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | O |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | Saint Joseph's University |
| Annotation Status | In GenBank |
| Plaque Notes | Clear, 1mm plaques. 48 hr incubation period. |
| Morphotype | Siphoviridae |
| Number of Genes | 127 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewSchuy_1 Schuy_2 Schuy_3 Schuy_4 Schuy_5 Schuy_6 Schuy_7 Schuy_8 Schuy_9 Schuy_10 Schuy_11 Schuy_12 Schuy_13 Schuy_14 Schuy_15 Schuy_16 Schuy_17 Schuy_18 Schuy_19 Schuy_20 Schuy_21 Schuy_22 Schuy_23 Schuy_24 Schuy_25 Schuy_26 Schuy_27 Schuy_28 Schuy_29 Schuy_30 Schuy_31 Schuy_32 Schuy_33 Schuy_34 Schuy_35 Schuy_36 Schuy_37 Schuy_38 Schuy_39 Schuy_40 Schuy_41 Schuy_42 Schuy_43 Schuy_44 Schuy_45 Schuy_46 Schuy_47 Schuy_48 Schuy_49 Schuy_50 Schuy_51 Schuy_52 Schuy_53 Schuy_54 Schuy_55 Schuy_56 Schuy_57 Schuy_58 Schuy_59 Schuy_60 Schuy_61 Schuy_62 Schuy_63 Schuy_64 Schuy_65 Schuy_66 Schuy_67 Schuy_68 Schuy_69 Schuy_70 Schuy_71 Schuy_72 Schuy_73 Schuy_74 Schuy_75 Schuy_76 Schuy_77 Schuy_78 Schuy_79 Schuy_80 Schuy_81 Schuy_82 Schuy_83 Schuy_84 Schuy_85 Schuy_86 Schuy_87 Schuy_88 Schuy_89 Schuy_90 Schuy_91 Schuy_92 Schuy_93 Schuy_94 Schuy_95 Schuy_96 Schuy_97 Schuy_98 Schuy_99 Schuy_100 Schuy_101 Schuy_102 Schuy_103 Schuy_104 Schuy_105 Schuy_106 Schuy_107 Schuy_108 Schuy_109 Schuy_110 Schuy_111 Schuy_112 Schuy_113 Schuy_114 Schuy_115 Schuy_116 Schuy_117 Schuy_118 Schuy_119 Schuy_120 Schuy_121 Schuy_122 Schuy_123 Schuy_124 Schuy_125 Schuy_126 Schuy_127 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP978896 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 166 |
| Pitt Freezer Box Grid# | F10 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |