| Detailed Information for Phage Sham |
| Discovery Information |
| Isolation Host | Streptomyces griseus ATCC 10137 |
| Found By | Amethyst Sauceda |
| Year Found | 2020 |
| Location Found | Denton, TX United States |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 33.235184 N, 97.158531 W Map |
| Discovery Notes | The temperature was around 96°F and it was slightly humid from oncoming rain. The location is on the edge of a lake. I dug from the edge of the lake with a spoon and got around 2in deep before placing the soil into my bag. |
| Naming Notes | My best friends are named Sam and Sam, and my nickname is Ham. We call our little group Sham, so I wanted to name my phage after us. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 22, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 415 |
| Genome length (bp) | 130327 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 787 bp |
| GC Content | 47.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BK |
| Subcluster | BK1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques range in size from <1mm to 1mm, and vary between comet and circular shapes. |
| Number of Genes | 224 |
| Number of tRNAs | 35 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON645339 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
118 |
| Pitt Freezer Box Grid# |
A5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
