Streptomyces phage Sham
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Detailed Information for Phage Sham
Discovery Information
Isolation HostStreptomyces griseus ATCC 10137
Found ByAmethyst Sauceda
Year Found2020
Location FoundDenton, TX United States
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates33.235184 N, 97.158531 W Map
Discovery NotesThe temperature was around 96°F and it was slightly humid from oncoming rain. The location is on the edge of a lake. I dug from the edge of the lake with a spoon and got around 2in deep before placing the soil into my bag.
Naming NotesMy best friends are named Sam and Sam, and my nickname is Ham. We call our little group Sham, so I wanted to name my phage after us.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 22, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage415
Genome length (bp)130327
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length787 bp
GC Content47.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBK
SubclusterBK1
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUniversity of North Texas
Annotation StatusIn GenBank
Plaque NotesPlaques range in size from <1mm to 1mm, and vary between comet and circular shapes.
Number of Genes224
Number of tRNAs35
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

Click to View

Sham_1

Sham_2

Sham_3

Sham_4

Sham_5

Sham_6

Sham_7
(DNA methyltransferase)

Sham_8
(ThyX-like thymidylate synthase)

Sham_9

Sham_10

Sham_11

Sham_12 — tRNA
(tRNA-Gly(ggt))

Sham_13 — tRNA
(tRNA-Arg(cgt))

Sham_14 — tRNA
(tRNA-Cys(tgt))

Sham_15

Sham_16
(glycosyltransferase)

Sham_17
(glycosyltransferase)

Sham_18
(ribonucleotide reductase)

Sham_19
(endolysin)

Sham_20

Sham_21

Sham_22
(portal protein)

Sham_23

Sham_24
(capsid maturation protease)

Sham_25
(major capsid protein)

Sham_26

Sham_27

Sham_28

Sham_29
(head-to-tail adaptor)

Sham_30

Sham_31

Sham_32

Sham_33
(major tail protein)

Sham_34
(tail assembly chaperone)

Sham_35
(tail assembly chaperone)

Sham_36
(tape measure protein)

Sham_37
(minor tail protein)

Sham_38
(minor tail protein)

Sham_39

Sham_40
(minor tail protein)

Sham_41
(minor tail protein)

Sham_42

Sham_43

Sham_44

Sham_45
(HNH endonuclease)

Sham_46
(metallophosphoesterase)

Sham_47

Sham_48
(helix-turn-helix DNA binding domain protein)

Sham_49

Sham_50

Sham_51

Sham_52
(DnaB-like dsDNA helicase)

Sham_53

Sham_54
(DNA primase)

Sham_55
(ssDNA binding protein)

Sham_56
(HNH endonuclease)

Sham_57
(DnaE-like DNA polymerase III alpha)

Sham_58

Sham_59
(MazG-like nucleotide pyrophosphohydrolase)

Sham_60

Sham_61
(RecA-like DNA recombinase)

Sham_62

Sham_63

Sham_64

Sham_65

Sham_66

Sham_67

Sham_68

Sham_69
(Cas4 family exonuclease)

Sham_70

Sham_71
(RuvC-like resolvase)

Sham_72
(DprA-like DNA processing chain A)

Sham_73

Sham_74
(methyltransferase)

Sham_75
(exonuclease)

Sham_76
(deoxynucleoside monophosphate kinase)

Sham_77
(5' nucleotidase)

Sham_78

Sham_79

Sham_80

Sham_81

Sham_82
(terminase)

Sham_83

Sham_84

Sham_85 — tRNA
(tRNA-Trp(tgg))

Sham_86
(HNH endonuclease)

Sham_87 — tRNA
(tRNA-Trp(tgg))

Sham_88
(HNH endonuclease)

Sham_89 — tRNA
(tRNA-Leu(ctg))

Sham_90 — tRNA
(tRNA-Pro(cca))

Sham_91

Sham_92 — tRNA
(tRNA-Asp(gat))

Sham_93 — tRNA
(tRNA-Ser(tga))

Sham_94

Sham_95 — tRNA
(tRNA-Val(gtc))

Sham_96

Sham_97 — tRNA
(tRNA-Ser(cga))

Sham_98

Sham_99

Sham_100
(Ro-like RNA binding protein)

Sham_101

Sham_102

Sham_103 — tRNA
(tRNA-Thr(acg))

Sham_104 — tRNA
(tRNA-Ser(tct))

Sham_105

Sham_106

Sham_107

Sham_108

Sham_109

Sham_110 — tRNA
(tRNA-Cys(tgc))

Sham_111

Sham_112 — tRNA
(tRNA-Leu(ctt))

Sham_113

Sham_114

Sham_115 — tRNA
(tRNA-Met(cat))

Sham_116 — tRNA
(tRNA-Met(cat))

Sham_117 — tRNA
(tRNA-Phe(gaa))

Sham_118

Sham_119

Sham_120

Sham_121

Sham_122 — tRNA
(tRNA-Val(gta))

Sham_123 — tRNA
(tRNA-Val(gtg))

Sham_124 — tRNA
(tRNA-Ala(gca))

Sham_125 — tRNA
(tRNA-Val(gtt))

Sham_126 — tRNA
(tRNA-Ser(tcg))

Sham_127

Sham_128 — tRNA
(tRNA-Pro(cct))

Sham_129 — tmRNA
(Tag peptide: ANSKNASAYALAA*)

Sham_130

Sham_131
(band-7-like membrane protein)

Sham_132

Sham_133

Sham_134

Sham_135

Sham_136 — tRNA
(tRNA-Glu(gag))

Sham_137

Sham_138

Sham_139

Sham_140 — tRNA
(tRNA-Ser(gct))

Sham_141
(RNA ligase)

Sham_142

Sham_143

Sham_144
(polynucleotide kinase)

Sham_145

Sham_146

Sham_147

Sham_148 — tRNA
(tRNA-Leu(tag))

Sham_150

Sham_151

Sham_152

Sham_153 — tRNA
(tRNA-Tyr(tac))

Sham_154 — tRNA
(tRNA-Gln(caa))

Sham_155

Sham_156

Sham_157

Sham_158

Sham_159

Sham_160

Sham_161

Sham_162
(metallophosphoesterase)

Sham_163

Sham_164 — tRNA
(tRNA-Phe(ttt))

Sham_165

Sham_166
(WhiB family transcription factor)

Sham_167

Sham_168

Sham_169

Sham_170 — tRNA
(tRNA-Tyr(tat))

Sham_171

Sham_172

Sham_173

Sham_174 — tRNA
(tRNA-Leu(ctc))

Sham_175 — tRNA
(tRNA-Phe(ttc))

Sham_176 — tRNA
(tRNA-Ala(gcc))

Sham_177

Sham_178

Sham_179 — tRNA
(tRNA-Ser(tcc))

Sham_180

Sham_181
(HNH endonuclease)

Sham_182

Sham_183
(nuclease)

Sham_184

Sham_185

Sham_186

Sham_187

Sham_188

Sham_189

Sham_190

Sham_191
(thioredoxin)

Sham_192

Sham_193

Sham_194

Sham_195
(glycosylase)

Sham_196

Sham_197

Sham_198
(RNA ligase)

Sham_199

Sham_200

Sham_201

Sham_202

Sham_203

Sham_204

Sham_205

Sham_206

Sham_207

Sham_208
(Lsr2-like DNA bridging protein)

Sham_209

Sham_210

Sham_211

Sham_212

Sham_213

Sham_214

Sham_215

Sham_216

Sham_217

Sham_218

Sham_219

Sham_220

Sham_221
(hydrolase)

Sham_222

Sham_223

Sham_224

Sham_225

Sham_226

Sham_227
(phosphoesterase)

Sham_228
(SprT-like protease)

Sham_229

Sham_230

Sham_231

Sham_232

Sham_233

Sham_234

Sham_235

Sham_236

Sham_237

Sham_238

Sham_239

Sham_240

Sham_241

Sham_242

Sham_243

Sham_244

Sham_245

Sham_246

Sham_247

Sham_248

Sham_249

Sham_250

Sham_251

Sham_252

Sham_253

Sham_254

Sham_255

Sham_256

Sham_257

Sham_258

Sham_259

Sham_260

Sham_261

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionON645339
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 118
Pitt Freezer Box Grid# A5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload