| Detailed Information for Phage Sisko | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Max Ungrey |
| Year Found | 2022 |
| Location Found | Tampa, FL United States |
| Finding Institution | University of South Florida |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 32°C |
| GPS Coordinates | 28.061111 N, 82.421389 W Map |
| Discovery Notes | The phage was found in a marshy area near Shriners Children's Hospital. I took soil from a grassy area on the water's edge which emitted a strong musky odor. The humidity was 75% and there was no recent precipitation. |
| Naming Notes | The phage is named after Commander Benjamin Sisko from the TV show Star Trek: Deep Space Nine. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 28, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 798 |
| Genome length (bp) | 62197 |
| Character of genome ends | Circularly Permuted |
| GC Content | 69.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | DR |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | University of South Florida |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques range in size from pinprick size to a diameter of up to 2mm. The plaques are temperate, so they have cloudy edges. |
| Morphotype | Siphoviridae |
| Number of Genes | 84 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewSisko_1 Sisko_2 Sisko_3 Sisko_4 Sisko_5 Sisko_6 Sisko_7 Sisko_8 Sisko_9 Sisko_10 Sisko_11 Sisko_12 Sisko_13 Sisko_14 Sisko_15 Sisko_16 Sisko_17 Sisko_18 Sisko_19 Sisko_20 Sisko_21 Sisko_22 Sisko_23 Sisko_24 Sisko_25 Sisko_26 Sisko_27 Sisko_28 Sisko_29 Sisko_30 Sisko_31 Sisko_32 Sisko_33 Sisko_34 Sisko_35 Sisko_36 Sisko_37 Sisko_38 Sisko_39 Sisko_40 Sisko_41 Sisko_42 Sisko_43 Sisko_44 Sisko_45 Sisko_46 Sisko_47 Sisko_48 Sisko_49 Sisko_50 Sisko_51 Sisko_52 Sisko_53 Sisko_54 Sisko_55 Sisko_56 Sisko_57 Sisko_58 Sisko_59 Sisko_60 Sisko_61 Sisko_62 Sisko_63 Sisko_64 Sisko_65 Sisko_66 Sisko_67 Sisko_68 Sisko_69 Sisko_70 Sisko_71 Sisko_72 Sisko_73 Sisko_74 Sisko_75 Sisko_76 Sisko_77 Sisko_78 Sisko_79 Sisko_80 Sisko_81 Sisko_82 Sisko_83 Sisko_84 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OQ938590 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 5.71x10^9 PFU/mL |
| Pitt Freezer Box# | 149 |
| Pitt Freezer Box Grid# | F9 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
