Gordonia phage Sisko
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Detailed Information for Phage Sisko
Discovery Information
Isolation HostGordonia terrae 3612
Found ByMax Ungrey
Year Found2022
Location FoundTampa, FL United States
Finding InstitutionUniversity of South Florida
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature32°C
GPS Coordinates28.061111 N, 82.421389 W Map
Discovery NotesThe phage was found in a marshy area near Shriners Children's Hospital. I took soil from a grassy area on the water's edge which emitted a strong musky odor. The humidity was 75% and there was no recent precipitation.
Naming NotesThe phage is named after Commander Benjamin Sisko from the TV show Star Trek: Deep Space Nine.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 28, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage798
Genome length (bp)62197
Character of genome endsCircularly Permuted
GC Content69.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterDR
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUniversity of South Florida
Annotation StatusIn GenBank
Plaque NotesPlaques range in size from pinprick size to a diameter of up to 2mm. The plaques are temperate, so they have cloudy edges.
MorphotypeSiphoviridae
Number of Genes84
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Sisko_1
(ParB-like nuclease domain protein)

Sisko_2
(ATP-binding cassette-like protein)

Sisko_3
(tRNA-methyltransferase)

Sisko_4
(terminase)

Sisko_5
(RuvC-like resolvase)

Sisko_6

Sisko_7
(portal protein)

Sisko_8
(capsid maturation protease)

Sisko_9

Sisko_10
(HNH endonuclease)

Sisko_11
(major capsid hexamer protein)

Sisko_12
(major capsid pentamer protein)

Sisko_13

Sisko_14

Sisko_15

Sisko_16

Sisko_17

Sisko_18
(major tail protein)

Sisko_19
(head-to-tail adaptor)

Sisko_20

Sisko_21

Sisko_22

Sisko_23
(tail assembly chaperone)

Sisko_24
(tape measure protein)

Sisko_25
(minor tail protein)

Sisko_26
(minor tail protein)

Sisko_27
(minor tail protein)

Sisko_28

Sisko_29

Sisko_30

Sisko_31

Sisko_32

Sisko_33

Sisko_34

Sisko_35
(hydrolase)

Sisko_36

Sisko_37

Sisko_38
(DNA binding protein)

Sisko_39
(helix-turn-helix DNA binding domain protein)

Sisko_40

Sisko_41
(lysin A)

Sisko_42
(lysin B)

Sisko_43

Sisko_44
(Cas4 exonuclease)

Sisko_45
(DNA helicase)

Sisko_46

Sisko_47
(helix-turn-helix DNA binding domain protein)

Sisko_48
(DNA primase/polymerase/helicase)

Sisko_49
(DNA polymerase I)

Sisko_50

Sisko_51

Sisko_52

Sisko_53

Sisko_54

Sisko_55
(polynucleotide kinase)

Sisko_56

Sisko_57

Sisko_58

Sisko_59

Sisko_60
(ribbon-helix-helix binding domain protein)

Sisko_61

Sisko_62
(helix-turn-helix DNA binding domain protein)

Sisko_63

Sisko_64

Sisko_65

Sisko_66

Sisko_67

Sisko_68

Sisko_69

Sisko_70

Sisko_71
(DNA binding protein)

Sisko_72
(HicA-like toxin)

Sisko_73

Sisko_74

Sisko_75

Sisko_76

Sisko_77

Sisko_78

Sisko_79

Sisko_80

Sisko_81

Sisko_82

Sisko_83

Sisko_84

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOQ938590
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 5.71x10^9 PFU/mL
Pitt Freezer Box# 149
Pitt Freezer Box Grid# F9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload