| Detailed Information for Phage Tophat |
| Discovery Information |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Anna Stowe Evans |
| Year Found | 2015 |
| Location Found | Lewisburg, PA USA |
| Finding Institution | Bucknell University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 28°C |
| GPS Coordinates | 40.956 N, 76.883 W Map |
| Discovery Notes | From a shady, fairly moist soil, removed top inch of soil before collected sample, under a redbud tree on Bucknell Campus
image of gel is undigested, BamHI, ClaI, EcoR1 and HinD3, Hae3 was run separately on a 2% gel |
| Naming Notes | I like tophats |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 23, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 3009 |
| Genome length (bp) | 70091 |
| Character of genome ends | Circularly Permuted |
| GC Content | 61.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AR |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | small, clear plaques |
| Number of Genes | 111 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF185725 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
3 x 10^9 |
| Date of SEA Lysate Titering |
Dec 3, 2015 |
| Pitt Freezer Box# |
22 |
| Pitt Freezer Box Grid# |
B12 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
