| Discovery Information |
| Isolation Host | Streptomyces scabiei RL-34 |
| Found By | Baily Kakacek |
| Year Found | 2021 |
| Location Found | Bel Air, MD United States |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.58972 N, 76.28048 W Map |
| Discovery Notes | Found outside of residential house in soil that had been unearthed from approximately 1 foot deep; the area where sample was taken was sprayed with Japanese beetle pesticide a day earlier. Weather was clear, moderate; sample taken midday on 9/12/21 at 28 C. |
| Naming Notes | "Vorvolakos" is a derivative of "Vrykolakas," the Romanized version of the Greek word for "vampire." The phage hijacks bacteria's inner workings or life force in a way to make more of itself--similar to how vampires do. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 3, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 13943 |
| Genome length (bp) | 46174 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 253 bp |
| GC Content | 60.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BF |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Appears to be virulent. Lysogeny test did not see any growth of additional cells, and remaining colonies in lysed spots of spot titer suspected to be immune. |
| Other Cluster Members |
|
| Annotating Institution | University of Maryland, Baltimore County |
| Annotation Status | In GenBank |
| Plaque Notes | ~1 mm in diameter, clear but only appearing turbid based on size |
| Morphotype | Podoviridae |
| Number of Genes | 67 |
| Number of tRNAs | 21 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON108647 |
| Refseq Number | None yet |
| Published in Paper | Davis et al 2022, Microbiology Resource Announcements: Genome Sequences of Streptomyces Bacteriophages Fabian, FlowerPower, Geostin, RetrieverFever, and Vorvolakos |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
132 |
| Pitt Freezer Box Grid# |
D1 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
