| Detailed Information for Phage Wakanda |
| Discovery Information |
| Isolation Host | Streptomyces sanglieri UNT16F27A |
| Found By | Ketsia Kankolongo |
| Year Found | 2019 |
| Location Found | Lewisville, TX United States |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 32.9901 N, 96.9681 W Map |
| Discovery Notes | The new soil sample was very moist. It was collected under the weather of -1 degrees Celsius. The plaque was donated by a classmate named Arti Mamidi. |
| Naming Notes | I liked the movie black panther and really liked the fictional place Wakanda. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 1, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 600 |
| Genome length (bp) | 126614 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 1773 bp |
| GC Content | 52.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BK |
| Subcluster | BK2 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | In my lysogeny test, I received negative results. My plaques showed infection
This means my phage is a virulent/lytic phage. |
| Other Cluster Members |
|
| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | My plaques are clear circles, and the size is 1.5 mm. |
| Number of Genes | 228 |
| Number of tRNAs | 34 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT024865 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
104 |
| Pitt Freezer Box Grid# |
D7 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
