Mycobacterium phage Wogge42
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| Detailed Information for Phage Wogge42 | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Macy Wente and Delaney Rogge |
| Year Found | 2024 |
| Location Found | Kenosha, WI United States |
| Finding Institution | Carthage College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 42.619 N, 87.822 W Map |
| Discovery Notes | The phage was discovered on Carthage College campus on a flower bed outside of the Student Union building. The soil was pretty surface level and not dug deep to get. The temperature outside was 72 degrees. |
| Naming Notes | The name for this phage came from a combination of both of our last names as well as our jersey numbers (27 + 15) added together. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 20, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 5898 |
| Genome length (bp) | 70899 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 4 bases |
| Overhang Sequence | GTGT |
| GC Content | 65.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | O |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | The consistently clear plaques lead us to believe that our phage is lytic. |
| Other Cluster Members | |
| Annotating Institution | Carthage College |
| Annotation Status | Being Annotated (Expected completion by 5/1/2025) |
| Plaque Notes | We consistently had plaques that were very small in diameter, circular, and clear. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 8.3 x 10^10 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
