Gordonia phage Yago84
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| Detailed Information for Phage Yago84 | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Former names | Tau19 |
| Found By | Jeremy Alwine |
| Year Found | 2016 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.438256 N, 79.948667 W Map |
| Discovery Notes | Yago84 was discovered in soil collected from Schenley Park in Pittsburgh, PA. The soil conditions were organic and moist, with weeds growing nearby. The weather at the time (3:10 PM) was sunny with calm winds and the temperature was 82oF. ~1/4 cup of soil was collected from a hole dug 3 inches into the soil that was 2 feet from the nearest plant. The soil sample was transported for ~30 minutes prior to storage in a refrigerator at 37oF. |
| Naming Notes | The name "Yago84" is formed from putting together the results of a random name generator ("Yago") and the results of a random number generator ("84"). |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 15, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2874 |
| Genome length (bp) | 61890 |
| Character of genome ends | Circularly Permuted |
| GC Content | 70.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | DR |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | The turbid appearance of some plaques produced by Yago84 suggests this phage may be temperate. As of yet, no lysogen testing has been performed to confirm this. |
| Other Cluster Members | |
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Yago84 produces a mixed morphology of cloudy plaques, ranging from 1 mm to 3 mm in diameter. Some of the Yago84 plaques are round, while others are more oval-shaped, though this may be a function of how the top agar was poured. |
| Morphotype | Siphoviridae |
| Number of Genes | 83 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK801725 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 6*10^9 pfu/mL |
| Date of SEA Lysate Titering | Oct 12, 2016 |
| Pitt Freezer Box# | 43 |
| Pitt Freezer Box Grid# | A2 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
