Mycobacterium phage Bogie
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Detailed Information for Phage Bogie
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesSpook
Found ByReid Rogers
Year Found2015
Location FoundCamden, AR USA
Finding InstitutionOuachita Baptist University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates33.470016 N, 92.873349 W Map
Discovery NotesDepth: 1.27 cm

Moist soil

Temp: 33.8889 Celsius

Location: Latitude: 33.4700165
Longitude: -92.87334859
Sea Level: 67 m

Weather:
24 August 2015 - 30 August 2015

Temperature:
Max- 33.8889 degrees Celsius
Mean- 23.8889 degrees Celsius
Min- 13.8889 degrees Celsius

Precipitation:
Rained 1.1938 cm on 24 August 2015
25 August 2015 - 30 August 2015 no precipitation occured.


Soil Sample Collection Information:
. On a North facing slope
. 20.32 cm above a pool of stagnant water with film on surface
. Flowing creek 243.84 cm from sample location
. Collected under green moss with red buds that released a fine red powder
. Collected below an oak tree and holly tree
. Just above sample site a decomposing oak tree with fungus is present
. No human traffic in proximity of sample site
. Deer and raccoon traffic around sample site
. Asphalt road approximately 32 meters from collection site

Naming NotesNamed after the term ghost in phages. i.e(synonym for ghost)
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 5, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2988
Genome length (bp)48639
Character of genome ends3' Sticky Overhang
Overhang Length12 bp
Overhang SequenceCCCGCCCCCCGA
GC Content66.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterP
SubclusterP1
Other Cluster Members
Plaque NotesSmall circular plaques with a large halo on the exterior. Hazy center and cloudy edges. Temperate phage resemblance.
MorphotypeSiphoviridae
Final annotation complete?Yes
Has been Phamerated?Yes
Gene List

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Bogie_1
(terminase, small subunit)

Bogie_2
(terminase, large subunit)

Bogie_3
(portal protein)

Bogie_4
(capsid maturation protease)

Bogie_5
(scaffolding protein)

Bogie_6
(major capsid protein)

Bogie_7
(head-to-tail connector protein)

Bogie_8
(head-to-tail connector protein)

Bogie_9
(head-to-tail connector protein)

Bogie_10
(head-to-tail connector protein)

Bogie_11
(head-to-tail connector protein)

Bogie_12
(head-to-tail connector protein)

Bogie_13
(major tail protein)

Bogie_14
(tail assembly chaperone)

Bogie_15
(tail assembly chaperone)

Bogie_16
(tape measure protein)

Bogie_17
(minor tail protein)

Bogie_18
(minor tail protein)

Bogie_19
(minor tail protein)

Bogie_20
(minor tail protein)

Bogie_21
(minor tail protein, D-ala-D-ala carboxypeptidase)

Bogie_22

Bogie_23

Bogie_24

Bogie_25

Bogie_26

Bogie_27

Bogie_28
(lysin A)

Bogie_29
(lysin B)

Bogie_30

Bogie_31

Bogie_32
(integrase)

Bogie_33
(immunity repressor)

Bogie_34
(helix-turn-helix DNA binding domain)

Bogie_35

Bogie_36
(antirepressor)

Bogie_37

Bogie_38

Bogie_39

Bogie_40

Bogie_41

Bogie_42

Bogie_43

Bogie_44

Bogie_45

Bogie_46

Bogie_47
(HNH endonuclease)

Bogie_48

Bogie_49
(RecE exonuclease)

Bogie_50
(RecT ssDNA binding protein)

Bogie_51

Bogie_52

Bogie_53
(FtsK DNA translocase)

Bogie_54
(RusA resolvase (endonuclease))

Bogie_55

Bogie_56

Bogie_57
(DNA methylase)

Bogie_58

Bogie_59

Bogie_60

Bogie_61
(helix-turn-helix DNA binding domain)

Bogie_62

Bogie_63

Bogie_64

Bogie_65

Bogie_66

Bogie_67

Bogie_68

Bogie_69

Bogie_70

Bogie_71

Bogie_72
(peptidase)

Bogie_73

Bogie_74

Bogie_75

Bogie_76

Bogie_77

Bogie_78

Bogie_79

Bogie_80

Bogie_81

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF133446
Refseq NumberNone yet
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload