| Detailed Information for Phage Dynamo | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Christina Holder, Kaitlyn Menard, Brady Tyler |
| Year Found | 2021 |
| Location Found | Longview, TX United States |
| Finding Institution | LeTourneau University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 32.46402 N, 94.728226 W Map |
| Discovery Notes | At 9:56 AM CET on 8/26/2021, our lab group (Christina Holder, Kaitlyn Menard, and Brady Tyler) gathered a soil sample beneath a tree near the west-central door of the Glaske Center at LeTourneau University. Brady Tyler used a plastic spoon to dig out an area approximately 8.2 cm x 6.3cm wide and 5.5 cm deep to collect 12 mL of soil. The weather was sunny with clear skies, and the temperature was approximately 29.4°C. The soil was dry and had small, dried-out roots around the dig site. |
| Naming Notes | Our phage was named after the prior complications we experienced with incubation and temperature control. Several of the plates resulting from purifying the phage were heat-shocked during incubation, and we had to start the process over multiple times. A 'Dynamo' is an object that generates force from heat transfer, so we decided that it fit well with the theme of our isolation and research of our phage. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 1, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1499 |
| Genome length (bp) | 46673 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | CCCGCCCCCCGA |
| GC Content | 67.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | P |
| Subcluster | P1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to ViewArib1 (P1) Atcoo (P1) Bartholomew (P1) Bogie (P1) Brusacoram (P1) Bunnies (P1) CactusJack (P1) Camster (P1) Donovan (P1) Dynamo (P1) FirstPlacePfu (P1) Fishburne (P1) Glaske (P1) GreaseLightnin (P1) HUHilltop (P1) Jebeks (P1) Jung (P1) Juniormint (P1) Kari (P1) KilKor (P1) Ksquared (P1) Langerak (P1) Majeke (P1) Malithi (P1) Mangethe (P1) Megiddo (P1) Necropolis (P1) Phalm (P1) Phegasus (P1) Phineas (P1) Polkaroo (P1) Pygmy (P1) Shipwreck (P1) Sonah (P1) StevieRay (P1) StressBall (P1) Techage (P1) Thespis (P1) Venti (P1) Vidya (P1) Willsammy (P1) Zilizebeth (P1) Tortellini (P2) Xavia (P3) BigNuz (P4) Nazo (P4) Phayonce (P5) ThulaThula (P5) Purky (P6) |
| Annotating Institution | LeTourneau University |
| Annotation Status | In GenBank |
| Plaque Notes | The phage exhibited two distinct plaque morphologies, despite sufficient purification. The larger plaques had more crisp margins, and had an average diameter of 1.25 mm. The smaller plaques had more fuzzy edges, and they exhibited an average diameter of 0.612 mm. Both were turbid, and the overall average diameter of the plaques was 0.931 mm. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewDynamo_1 Dynamo_2 Dynamo_3 Dynamo_4 Dynamo_5 Dynamo_6 Dynamo_7 Dynamo_8 Dynamo_9 Dynamo_10 Dynamo_11 Dynamo_12 Dynamo_13 Dynamo_14 Dynamo_15 Dynamo_16 Dynamo_17 Dynamo_18 Dynamo_19 Dynamo_20 Dynamo_21 Dynamo_22 Dynamo_23 Dynamo_24 Dynamo_25 Dynamo_26 Dynamo_27 Dynamo_28 Dynamo_29 Dynamo_30 Dynamo_31 Dynamo_32 Dynamo_33 Dynamo_34 Dynamo_35 Dynamo_36 Dynamo_37 Dynamo_38 Dynamo_39 Dynamo_40 Dynamo_41 Dynamo_42 Dynamo_43 Dynamo_44 Dynamo_45 Dynamo_46 Dynamo_47 Dynamo_48 Dynamo_49 Dynamo_50 Dynamo_51 Dynamo_52 Dynamo_53 Dynamo_54 Dynamo_55 Dynamo_56 Dynamo_57 Dynamo_58 Dynamo_59 Dynamo_60 Dynamo_61 Dynamo_62 Dynamo_63 Dynamo_64 Dynamo_65 Dynamo_66 Dynamo_67 Dynamo_68 Dynamo_69 Dynamo_70 Dynamo_71 Dynamo_72 Dynamo_73 Dynamo_74 Dynamo_75 Dynamo_76 Dynamo_77 Dynamo_78 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OP434454 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 134 |
| Pitt Freezer Box Grid# | C12 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |