Microbacterium phage Scumberland
Scumberland Plaque Picture
Scumberland EM Picture
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Detailed Information for Phage Scumberland
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByMontana Heath Simpson
Year Found2019
Location FoundClarksville, TN United States
Finding InstitutionAustin Peay State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature21°C
GPS Coordinates36.541667 N, 87.367725 W Map
Discovery NotesTime of Day: 10:15 am
Location: Between the Waffle House and Electric Cowboy on Riverside Dr.
Description of Location: Below walking track and extracted from river bank.
Depth: 0.5 to 2.0 cm
Naming NotesI obtained the phage sample from an environmental sample which was extracted from the side of the river bank where the Cumberland River merges with the Red Rivers.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 23, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage73
Genome length (bp)53276
Character of genome endsCircularly Permuted
GC Content68.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEC
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionAustin Peay State University
Annotation StatusIn GenBank
Plaque NotesSmall plaque morphology manly lytic plaques have been observed, but occasional lysogeneic plaques also have been observed.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Scumberland_1
(terminase small subunit)

Scumberland_2

Scumberland_3

Scumberland_4
(terminase)

Scumberland_5

Scumberland_6

Scumberland_7

Scumberland_8

Scumberland_9

Scumberland_10

Scumberland_11

Scumberland_12

Scumberland_13

Scumberland_14

Scumberland_15

Scumberland_16

Scumberland_17

Scumberland_18

Scumberland_19

Scumberland_20

Scumberland_21
(portal protein)

Scumberland_22

Scumberland_23

Scumberland_24
(MuF-like minor capsid protein)

Scumberland_25

Scumberland_26
(capsid maturation protease)

Scumberland_27

Scumberland_28
(major capsid hexamer protein)

Scumberland_29

Scumberland_30

Scumberland_31
(major tail protein)

Scumberland_32
(head-to-tail adaptor)

Scumberland_33

Scumberland_34

Scumberland_35
(tail assembly chaperone)

Scumberland_36
(tail assembly chaperone)

Scumberland_37
(tape measure protein)

Scumberland_38
(minor tail protein)

Scumberland_39
(minor tail protein)

Scumberland_40
(minor tail protein)

Scumberland_41
(minor tail protein)

Scumberland_42

Scumberland_43

Scumberland_44
(lysin A)

Scumberland_45

Scumberland_46

Scumberland_47

Scumberland_48

Scumberland_49
(exonuclease)

Scumberland_50
(ERF family ssDNA binding protein)

Scumberland_51

Scumberland_52

Scumberland_53
(helix-turn-helix DNA binding domain protein)

Scumberland_54
(ADP-ribosyltransferase)

Scumberland_55
(HNH endonuclease)

Scumberland_56

Scumberland_57

Scumberland_58

Scumberland_59

Scumberland_60

Scumberland_61

Scumberland_62

Scumberland_63

Scumberland_64

Scumberland_65

Scumberland_66
(ssDNA-binding protein)

Scumberland_67

Scumberland_68
(helix-turn-helix DNA binding domain protein)

Scumberland_69
(helix-turn-helix DNA binding domain protein)

Scumberland_70

Scumberland_71

Scumberland_72

Scumberland_73

Scumberland_74

Scumberland_75

Scumberland_76

Scumberland_77

Scumberland_78

Scumberland_79

Scumberland_80

Scumberland_81

Scumberland_82

Scumberland_83

Scumberland_84
(RNA polymerase sigma factor)

Scumberland_85
(ParB-like nuclease domain protein)

Scumberland_86
(glycosyltransferase)

Scumberland_87
(UDP-glucose dehydrogenase)

Scumberland_88
(AAA-ATPase)

Scumberland_89

Scumberland_90

Scumberland_91

Scumberland_92

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT818415
Refseq NumberNone yet
Published in PaperMarkov et al 2021, Microbiology Resource Announcements: Complete Genome Sequences of Microbacterium Bacteriophages Danno, Otwor, and Scumberland, Isolated in Clarksville, Tennessee
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.0 x 10^9
Pitt Freezer Box# 97
Pitt Freezer Box Grid# H11
Available Files
GenBank File for PhameratorDownload