| Detailed Information for Phage Canowicakte |
| Discovery Information |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Conor Holton |
| Year Found | 2015 |
| Location Found | Radnor, PA USA |
| Finding Institution | Cabrini University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.014167 N, 75.393611 W Map |
| Discovery Notes | Found 2 miles into a forrest preserve by a marsh. Dug approx. 1 foot down into the soil |
| Naming Notes | Sioux term for Forrest Hunter. Celebrating native american heritage. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 17, 2015 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2872 |
| Genome length (bp) | 43914 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 13 bases |
| Overhang Sequence | GGTAACCGTGATA |
| GC Content | 61.2% |
| Sequencing Notes | A small percentage (~6%) of the reads in this project were from a Cluster AN phage, suggesting that perhaps there was some contamination or a non-pure sample. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AK |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Grows over 2 days in 30 degrees Celsius storage and forms clear plaques. Can grow at room temperature but not at 37 degrees Celsius. |
| Morphotype | Siphoviridae |
| Number of Genes | 62 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF140400 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
Cabrini College |
| SEA Lysate Titer |
4.8 X 10^7 pfu/ml |
| Date of SEA Lysate Titering |
Aug 5, 2015 |
| Pitt Freezer Box# |
149 |
| Pitt Freezer Box Grid# |
B9 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
