Arthrobacter phage Christian
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Detailed Information for Phage Christian
Discovery Information
Isolation HostArthrobacter sp. ATCC 21022
Found ByJewel Smith
Year Found2015
Location FoundWest Deptford, NJ USA
Finding InstitutionCabrini University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates39.8318 N, 75.1896 W Map
Discovery NotesThis soil sample was taken from under a tree at the park at my apartment complex.
Naming NotesThis soil sample was dug up from under a tree at the park with the assistance of my nephew Christian.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 17, 2015
Sequencing FacilityNC State Genomic Sciences Laboratory
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1818
Genome length (bp)43081
Character of genome ends3' Sticky Overhang
Overhang Length13 bases
Overhang SequenceGGTAACCGTGATA
GC Content60.7%
Fasta file available?Yes: Download fasta file
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesWith various Calcium concentrations experimented with, it was found that at room temperature and overnight incubation there are tiny but plentiful and clear plaques grown. At 30 degrees Celsius and overnight incubation there were bigger plaques but still small and clear but less plentiful. At 37 degrees Celsius and overnight incubation there is no growth at all. As the concentration of calcium increases the bigger the plaques grow. Calcium concentrations experimented with were: 0 mM, 5 mM, 25 mM, and 50 mM. At 30 degrees Celsius without calcium there is no growth.
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF140404
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator Cabrini College
SEA Lysate Titer 7.8 X 10^6
Date of SEA Lysate Titering Jul 1, 2015
Pitt Freezer Box# 149
Pitt Freezer Box Grid# B12
Available Files
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload