Arthrobacter phage Huckleberry
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Detailed Information for Phage Huckleberry
Discovery Information
Isolation HostArthrobacter sp. ATCC 21022
Found ByElena Golub & Alicia Williams
Year Found2016
Location FoundNorthampton, MA USA
Finding InstitutionSmith College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates42.3393 N, 72.6314 W Map
Discovery NotesSoil was dark in color with some mulch and dryer organic matter.
Naming NotesOur phage is an adventurer like Huckleberry Finn, and was found near the Connecticut River. Therefore, we named our phage Huckleberry.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 3, 2017
Sequencing FacilitySmith College
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage6297
Genome length (bp)42972
Character of genome ends3' Sticky Overhang
Overhang Length13 bases
Overhang SequenceGGTAACCGTGATA
GC Content61.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterAK
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesPlaques were large and mostly in close proximity. Our phage is lytic.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Huckleberry_1

Huckleberry_2
(terminase)

Huckleberry_3
(portal and MuF-like fusion protein)

Huckleberry_4
(capsid maturation protease)

Huckleberry_5
(major capsid protein)

Huckleberry_6

Huckleberry_7
(head-to-tail adaptor)

Huckleberry_8
(head-to-tail stopper)

Huckleberry_9

Huckleberry_10
(tail terminator)

Huckleberry_11
(major tail protein)

Huckleberry_12
(tail assembly chaperone)

Huckleberry_13
(tail assembly chaperone)

Huckleberry_14
(tape measure protein)

Huckleberry_15
(minor tail protein)

Huckleberry_16
(minor tail protein)

Huckleberry_17
(minor tail protein)

Huckleberry_18
(minor tail protein)

Huckleberry_19

Huckleberry_20

Huckleberry_21
(endolysin)

Huckleberry_22
(holin)

Huckleberry_23
(helix-turn-helix DNA binding domain protein)

Huckleberry_24

Huckleberry_25
(DNA helicase)

Huckleberry_26

Huckleberry_27

Huckleberry_28

Huckleberry_29

Huckleberry_30

Huckleberry_31

Huckleberry_32

Huckleberry_33

Huckleberry_34

Huckleberry_35

Huckleberry_36

Huckleberry_37

Huckleberry_38

Huckleberry_39
(Cas4 family exonuclease)

Huckleberry_40

Huckleberry_41
(RecA-like DNA recombinase)

Huckleberry_42

Huckleberry_43
(DNA primase/polymerase/helicase)

Huckleberry_44

Huckleberry_45
(DNA polymerase I)

Huckleberry_46
(PurA-like adenylosuccinate synthetase)

Huckleberry_47

Huckleberry_48

Huckleberry_49
(deoxyuridine triphosphatase)

Huckleberry_50

Huckleberry_51
(helix-turn-helix DNA binding domain protein)

Huckleberry_52

Huckleberry_53

Huckleberry_54

Huckleberry_55

Huckleberry_56

Huckleberry_57

Huckleberry_58

Huckleberry_59

Huckleberry_60

Huckleberry_61

Huckleberry_62
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK279856
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 32
Pitt Freezer Box Grid# D10
Available Files
Final DNAMaster FileDownload
GenBank File for PhameratorDownload