Arthrobacter phage OurGirlNessie
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Detailed Information for Phage OurGirlNessie
Discovery Information
Isolation HostArthrobacter sp. ATCC 21022
Former namesKickassDragon
Found ByJane Milcetic, Sarita Quimpo-Chiu, Shi Ying Feng
Year Found2016
Location FoundNorthampton, MA United States of America
Finding InstitutionSmith College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates42.319362 N, 72.641215 W Map
Discovery NotesDiscovered by the banks of Paradise Pond at Smith College. Soil is moist and chunky with some plant debris.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 6, 2017
Sequencing FacilitySmith College
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage4757
Genome length (bp)42561
Character of genome ends3' Sticky Overhang
Overhang Length13 bases
Overhang SequenceGGTAACCGTGATA
GC Content60.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterAK
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotation StatusQC Complete
Plaque NotesDespite having an assumed pure phage (the phage used here was initially from a single plaque), it is important to note that there are small and big plaques. The inconsistency in size may also be attributed to an uneven distribution of bacteria. We conclude that plaque morphology is consistent, and looks to be a pure sample.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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OurGirlNessie_1

OurGirlNessie_2
(terminase)

OurGirlNessie_3
(portal protein)

OurGirlNessie_4
(capsid maturation protease)

OurGirlNessie_5
(major capsid protein)

OurGirlNessie_6

OurGirlNessie_7
(head-to-tail adaptor)

OurGirlNessie_8
(head-to-tail stopper)

OurGirlNessie_9

OurGirlNessie_10
(tail terminator)

OurGirlNessie_11
(major tail protein)

OurGirlNessie_12
(tail assembly chaperone)

OurGirlNessie_13
(tail assembly chaperone)

OurGirlNessie_14
(tape measure protein)

OurGirlNessie_15
(minor tail protein)

OurGirlNessie_16
(minor tail protein)

OurGirlNessie_17
(minor tail protein)

OurGirlNessie_18
(minor tail protein)

OurGirlNessie_19

OurGirlNessie_20

OurGirlNessie_21
(lysin A)

OurGirlNessie_22
(holin)

OurGirlNessie_23
(helix-turn-helix DNA binding domain)

OurGirlNessie_24

OurGirlNessie_25
(DNA helicase)

OurGirlNessie_26

OurGirlNessie_27

OurGirlNessie_28

OurGirlNessie_29

OurGirlNessie_30

OurGirlNessie_31

OurGirlNessie_32

OurGirlNessie_33

OurGirlNessie_34

OurGirlNessie_35

OurGirlNessie_36

OurGirlNessie_37

OurGirlNessie_38
(DNA helicase)

OurGirlNessie_39

OurGirlNessie_40
(RecA-like DNA recombinase)

OurGirlNessie_41

OurGirlNessie_42
(DNA primase/ polymerase)

OurGirlNessie_43

OurGirlNessie_44

OurGirlNessie_45
(adenylsuccinate synthetase)

OurGirlNessie_46

OurGirlNessie_47

OurGirlNessie_48
(deoxyuridine triphosphatase)

OurGirlNessie_49

OurGirlNessie_50
(helix-turn-helix DNA binding domain)

OurGirlNessie_51

OurGirlNessie_52
(DNA binding protein)

OurGirlNessie_53

OurGirlNessie_54

OurGirlNessie_55

OurGirlNessie_56

OurGirlNessie_57

OurGirlNessie_58

OurGirlNessie_59
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 32
Pitt Freezer Box Grid# E8
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload