Arthrobacter phage Eunoia
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Detailed Information for Phage Eunoia
Discovery Information
Isolation HostArthrobacter sp. 68b
Found ByDesislava Stoyanova & Asia DeRosby
Year Found2017
Location FoundNorthampton, MA USA
Finding InstitutionSmith College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS Coordinates42.3180 N, 72.6450 W Map
Discovery NotesDate/Time: 09/18/2017 at 11:15am

Weather: 19℃; cloudy, foggy and drizzling earlier

Description of sample site: Botanical Garden-Compost

Soil description: rather dry on top, moist about 10.5 cm below top surface; grayish brown, mixed with some mulch; it is compost from Fork Hill, about 8 years old and primarily consists of leaves

Depth of sample site: ~ 28cm

Naming NotesPronunciation: yoo'-noy-ah

Meaning: a well mind; beautiful thinking
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 8, 2018
Sequencing FacilitySmith College
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage4750
Genome length (bp)44416
Character of genome ends3' Sticky Overhang
Overhang Length13 bases
Overhang SequenceGCTTACCGTGATA
GC Content61.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterAK
Subcluster--
Cluster Life CycleLytic
Lysogeny NotesLYTIC PHAGES

Due to the plaque morphology being clear, easy to see, round plaques, it leads us to believe that our plaques are from lytic phages, since they match the description of lytic phage plaques precisely.

Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesPlaque Size: ~1-2 ml, fairly small

Clear plaques with clear edges.

Plaque shapes were clearly define.

All plaques were very circular in shape.

Dispersed across plate, not highly concentrated in one area of the plate.

None of the other plates had plaques present.


MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK279851
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 3.6 x 10^4
Pitt Freezer Box# 58
Pitt Freezer Box Grid# G11
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload