| Detailed Information for Phage DannyDe |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Eric Nordberg and Gianna Moscatelli |
| Year Found | 2019 |
| Location Found | Philadelphia, PA United States |
| Finding Institution | University of the Sciences (now St. Joseph's Univ) |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 23°C |
| GPS Coordinates | 39.94521 N, 75.208113 W Map |
| Discovery Notes | This soil sample was collected outside of the McNeil Science And Technology Center at University of the Sciences in Philadelphia. This soil sample was taken from the smartest dirt at a depth of 2cm. |
| Naming Notes | We named this phage DannyDe because the soil sample was taken only 15 minutes from the show it's always sunny in Philadelphia, who one of the main actors name is DannyDe |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 20, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 49 |
| Genome length (bp) | 56087 |
| Character of genome ends | Circularly Permuted |
| GC Content | 63.6% |
| Sequencing Notes | The phage was sequenced as part of a DOGEMS sample. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EF |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Salish Kootenai College |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 83 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW055903 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.43*10^7 |
| Pitt Freezer Box# |
98 |
| Pitt Freezer Box Grid# |
C10 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
