| Detailed Information for Phage Dennebes |
| Discovery Information |
| Isolation Host | Streptomyces mirabilis NRRL B-2400 |
| Found By | Junior Mkpasi, Phebe Ismail |
| Year Found | 2022 |
| Location Found | Westminster, MD United States |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 71°C |
| GPS Coordinates | 39.5 N, 77.0 W Map |
| Discovery Notes | Collected by Junior Mkpasi on 9/12/22 from a wet soil rich in organic matter. |
| Naming Notes | The naming came from the joining together of my partner's name (Dennis) and my name (Phebe). |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 23, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1735 |
| Genome length (bp) | 46162 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 254 bp |
| GC Content | 60.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BF |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | After testing for lysogen, the results showed that no lysogen was formed. |
| Other Cluster Members |
|
| Annotating Institution | University of Maryland, Baltimore County |
| Annotation Status | In GenBank |
| Plaque Notes | ~ 1mm in diameter, cloudy and round after 48 hours at 30 C. on S. mirabilis. |
| Morphotype | Podoviridae |
| Number of Genes | 68 |
| Number of tRNAs | 23 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR253913 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
5 x 10^11 pfu/ml |
| Pitt Freezer Box# |
151 |
| Pitt Freezer Box Grid# |
B3 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| Virtual Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
