Mycobacterium phage Dylan
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| Detailed Information for Phage Dylan | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Vanisha Munsamy |
| Year Found | 2011 |
| Location Found | Durban, South Africa |
| Finding Institution | University of Kwazulu-Natal |
| Program | Mycobacterial Genetics Course, Durban, South Africa |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 29.8587 S, 31.0218 E Map |
| Discovery Notes | Found in moist soil on the property of 472 Grove End Drive, Phoenix, Durban, South Africa. Soil sample taken from 2-3cm deep ground. Temperature approximately 20-25 degrees Celsius. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jun 29, 2012 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Ion Torrent |
| Approximate Shotgun Coverage | 865 |
| Sanger Finishing Reactions | 30 |
| Genome length (bp) | 69815 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 4 bases |
| Overhang Sequence | GTGT |
| GC Content | 65.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | O |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques approximately 0.2 mm in diameter, small and round in shape. Turbid plaques were observed. 5 phage particles were measured using a Transmission Election Microscope. The average morphology is as follows: Head width: 32.1nm, head length: 142.8nm, tail length: 233.68nm. |
| Morphotype | Siphoviridae |
| Number of Genes | 121 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KF024730 |
| Refseq Number | NC_022325 |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 1 |
| Pitt Freezer Box Grid# | G1 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |