| Discovery Information |
| Isolation Host | Streptomyces scabiei RL-34 |
| Former names | GreenThumb |
| Found By | Olivia Richter, Jessica Sprando |
| Year Found | 2017 |
| Location Found | Catonsville, MD USA |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.256894 N, 76.714128 W Map |
| Discovery Notes | Temperature 30°C from moist soil collected ~3 inches from surface in UMBC Garden |
| Naming Notes | The origin of the name is a reference to gardening in general since the sample was collected from a garden. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 2, 2018 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Genome length (bp) | 46133 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 253 bp |
| GC Content | 60.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BF |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Maryland, Baltimore County |
| Annotation Status | In GenBank |
| Plaque Notes | 1.5mm, round, turbid, infection in ~24 hours on S.scabiei in 30°C incubator |
| Morphotype | Podoviridae |
| Number of Genes | 66 |
| Number of tRNAs | 21 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH155868 |
| Refseq Number | None yet |
| Published in Paper | Davis et al 2022, Microbiology Resource Announcements: Genome Sequences of Streptomyces Bacteriophages Fabian, FlowerPower, Geostin, RetrieverFever, and Vorvolakos |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.36 x 10^9 |
| Pitt Freezer Box# |
49 |
| Pitt Freezer Box Grid# |
E12 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
