| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Nicole Bowen |
| Year Found | 2023 |
| Location Found | Glenside, PA United States |
| Finding Institution | Saint Joseph's University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.099589 N, 75.142356 W Map |
| Discovery Notes | Phage was collected on January 15th, 2023 at 4:15pm at Hallowell Park in Glenside, PA 19038. I dug in soil in a dried up creek bed that was about 50 feet from a baseball field and 150 feet from a fastener factory.
Soil condition- moist, saturated,sandy
Depth- 17.78 cm
Temperature- 0.6 C
Weather conditions- cold, cloudy, dry |
| Naming Notes | When I was digging in the soil of the dried up creek bed to collect my sample, I dug up 2 baseballs. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 18, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1174 |
| Genome length (bp) | 71074 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 4 bases |
| Overhang Sequence | GTGT |
| GC Content | 65.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | O |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Saint Joseph's University |
| Annotation Status | In GenBank |
| Plaque Notes | clear, 1mm plaques
Webbed plates exhibited 3/4 mm plaques
incubation length= 48 hours |
| Number of Genes | 126 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP978769 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
166 |
| Pitt Freezer Box Grid# |
E9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
