| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Thientrinh Nguyen |
| Year Found | 2021 |
| Location Found | Wynnewood, PA United States of America |
| Finding Institution | Saint Joseph's University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 39.998871 N, 75.28797 W Map |
| Discovery Notes | Idergollasper was found under a tree at a corner of South Ardmore Park between Sussex Road and Athens Avenue in Wynnewood, Pennsylvania. The soil sample was collected under a tree located closer to the side of Sussex Road by Julia Iacovella on Monday, January 25, 2021 at 3:00 pm. The depth of the soil was 13 cm, and the soil was hard and dry. The weather conditions when the soil sample was collected was cold and windy with overcast. The ambient temperature was 4.44 degrees Celsius and the ambient humidity was 50%.
The titer of the HTL is 1.7 x 10^9 pfu/mL. The concentration of Idergollasper's DNA is 203.0 ng/uL. The OD 260/280 ratio is 1.82.
The EM image shows that Idergollasper phage particles have a prolate capsid head and a non-contractile tail. Average Idergollasper phage particles measure 150 nm (capsid length), 25 nm (capsid width), and 275 nm (capsid tail), N=3.
Because Idergollasper's restriction digest is similar to other Cluster O's virtual digest, it is likely that Idergollasper belongs to Cluster O. |
| Naming Notes | Idergollasper is the name of a random playlist that was suggested to me on Spotify. Idergollasper is also my study playlist, and it was playing when I was collecting one of my soil sample. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 17, 2021 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 153 |
| Genome length (bp) | 72514 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 4 bases |
| Overhang Sequence | GTGT |
| GC Content | 65.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | O |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Saint Joseph's University |
| Annotation Status | In GenBank |
| Plaque Notes | Idergollasper forms small, clear plaques less than 1 mm in diameter with small, cloudy bumps surrounding each plaque. The plaques were formed after 24 to 72 hours in the incubator at 37 degrees Celsius. |
| Morphotype | Siphoviridae |
| Number of Genes | 131 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON260829 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
129 |
| Pitt Freezer Box Grid# |
H7 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
