| Detailed Information for Phage Lewando |
| Discovery Information |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Former names | Lewy |
| Found By | Maximilian Crosby |
| Year Found | 2022 |
| Location Found | Montreal, Canada |
| Finding Institution | McGill University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 9°C |
| GPS Coordinates | 45.51058 N, 73.56509 W Map |
| Discovery Notes | Lewando was isolated from the soil inside of a planter. This planter was located out in front of a metro station. The planter was exposed to the elements and was in direct sunlight. Additionally, the planter contained a variety of annual flowers and grasses. On the day of collection, conditions were overcast. The soil itself was slightly damp, as it had rained the day before. |
| Naming Notes | Lewando is named after a footballer, and will hopefully bring them luck in the current world cup (2022). |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 3, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2250 |
| Genome length (bp) | 55075 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGGCCT |
| GC Content | 51.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AU |
| Subcluster | AU6 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | McGill University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques appear clear in the middle, and turbid on the edges. Plaque size is variable. |
| Number of Genes | 89 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR553902 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
153 |
| Pitt Freezer Box Grid# |
D2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
