| Detailed Information for Phage Lifes |
| Discovery Information |
| Isolation Host | Microbacterium paraoxydans NRRL B-14843 |
| Former names | LiFES |
| Found By | Olivia Gronholz |
| Year Found | 2018 |
| Location Found | Green Isle, MN USA |
| Finding Institution | University of Wisconsin-River Falls |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 28°C |
| GPS Coordinates | 44.679121 N, 94.012799 W Map |
| Discovery Notes | Soil sample was very dry and clumpy. The dirt was loose.
Soil was taken from a newly planted tree; this is where multiple trees have died.
The soil was taken from a depth of 80 mm.
The temperature was 70 degrees F and it was sunny, not humid, and there was a breeze. |
| Naming Notes | The name is inspired by names in my life. There are some things with names that start with L,F,E, and S in my life. I decided to make it an acronym, becoming LiFES. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 27, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 392 |
| Genome length (bp) | 59253 |
| Character of genome ends | Direct Terminal Repeat |
| Overhang Length | 1350 bases |
| GC Content | 58.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | GB |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
|
| Annotating Institution | University of Wisconsin-River Falls |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques were all very clear. They had no halos and they were all uniform in size. |
| Morphotype | Siphoviridae |
| Number of Genes | 124 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT818426 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
87 |
| Pitt Freezer Box Grid# |
H6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
