| Detailed Information for Phage Lucy | |
| Discovery Information | |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Kate Starr |
| Year Found | 2015 |
| Location Found | Immaculata, PA USA |
| Finding Institution | Cabrini University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.013017 N, 75.605443 W Map |
| Discovery Notes | Soil sample was retrieved from under a tree and taken from a whole dug by a dog. |
| Naming Notes | Lucy is the name of the dog that dug the hole where the soil sample was taken from. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 17, 2015 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1947 |
| Genome length (bp) | 42944 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 13 bases |
| Overhang Sequence | GGTAACCGTGATA |
| GC Content | 60.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AK |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewAlbanese Aledel AppleCider AustinPowers Beethoven Bennie BigMack Bodacious BrotherBLo CallieOMalley Canowicakte Carpal ChewChew Cholula Chridison Christian CristinaYang Daiboju Dino DreamTeam DrRobert EstebanJulior Eunoia Fluke Gisselle Glenn GreenHearts Greenhouse HeadNerd Herb Huckleberry HunterDalle Huntingdon Immaculata Joann Jumboset Kalizoi KingBob Kittykat Korra Lakshmi Lasagna Lennox LilStuart Litotes Lucy Makoto MamaPearl Maria1952 MeganNoll Misaeng Moki MrGloopy Nancia Nubia OMalley OurGirlNessie Oxynfrius PartyCup PinkFriday PitaDog Potatoes Preamble Pterodactyl Pumancara RAP15 RcigaStruga Riovina Riverdale Rozby Savage2526 Scuttle Sergei Supakev Suppi TattModd Temper16 Urla Vallejo Vulture Wawa Wayne WonderBoy Zorro |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Lucy produces small, clear, round plaques. Plaque size remained consistent during the isolation process. Lucy has a fairly low titer. Optimal growth occurs between room temperature and 30 degrees Celsius. Does not grow at 37 degrees Celsius. Growth occurred with 0, 22.5, and 50 mM CaCl2 top agar. Also, Lucy grows better with MgSO4 rather than CaCl2. The plaques were bigger, and more robust in the varying conditions of MgSO4. The MgSO4 concentrations used were: 5, 25, and 50 mM. |
| Morphotype | Siphoviridae |
| Number of Genes | 60 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewLucy_1 Lucy_2 Lucy_3 Lucy_4 Lucy_5 Lucy_6 Lucy_7 Lucy_8 Lucy_9 Lucy_10 Lucy_11 Lucy_12 Lucy_13 Lucy_14 Lucy_15 Lucy_16 Lucy_17 Lucy_18 Lucy_19 Lucy_20 Lucy_21 Lucy_22 Lucy_23 Lucy_24 Lucy_25 Lucy_26 Lucy_27 Lucy_28 Lucy_29 Lucy_30 Lucy_31 Lucy_32 Lucy_33 Lucy_34 Lucy_35 Lucy_36 Lucy_37 Lucy_38 Lucy_39 Lucy_40 Lucy_41 Lucy_42 Lucy_43 Lucy_44 Lucy_45 Lucy_46 Lucy_47 Lucy_48 Lucy_49 Lucy_50 Lucy_51 Lucy_52 Lucy_53 Lucy_54 Lucy_55 Lucy_56 Lucy_57 Lucy_58 Lucy_59 Lucy_60 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX576641 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Designator | Cabrini College |
| SEA Lysate Titer | 2.4E7 |
| Date of SEA Lysate Titering | Jul 20, 2015 |
| Pitt Freezer Box# | 149 |
| Pitt Freezer Box Grid# | C9 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |