| Detailed Information for Phage NiebruSaylor |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Kaitlyn Niebrugge and Dalton Saylor |
| Year Found | 2019 |
| Location Found | West Lafayette, IN United States |
| Finding Institution | Purdue University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 19°C |
| GPS Coordinates | 40.47 N, 86.92 W Map |
| Discovery Notes | The dirt sample was collected from a shrub filled garden located next to a parking garage. It is worth noting that the dirt was collected next to a rather large mushroom. |
| Naming Notes | The phage name comes from our last names |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 13, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1170 |
| Genome length (bp) | 70777 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 4 bases |
| Overhang Sequence | GTGG |
| GC Content | 65.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | O |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Purdue University |
| Annotation Status | In GenBank |
| Plaque Notes | This phage forms quite small, circular plaques. The plaques are distinct circles, and are not cloudy. |
| Morphotype | Siphoviridae |
| Number of Genes | 127 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT818425 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
108 |
| Pitt Freezer Box Grid# |
B7 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
