| Detailed Information for Phage PinkFriday |
| Discovery Information |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Alexis Arredondo |
| Year Found | 2023 |
| Location Found | Santa Monica, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.042118 N, 118.444732 W Map |
| Discovery Notes | Isolated by TAs Dominic Garza and Michelle Zorawik.
Isolated using mixed culture method.
An enriched culture was prepared by combining 15 mL of soil with 25 mL of PYCA broth. The bacterial strains of A. sp., A. globiformis, A. sulfureus and A. atrocyaneus were then added in a ratio of 1:5:192:588 respectively. The enriched culture was incubated at 30℃ for 48 hours with shaking. After filtering with a 0.22 µm filter, the sample was plated on PYCA plates with 2X PYCa soft agar using the standard pour plate method.
Sample description: The soil samples was collected from an urban area with heavy foot traffic. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 19, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1991 |
| Genome length (bp) | 43295 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 13 bases |
| Overhang Sequence | GCTTACCGTGATA |
| GC Content | 61.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AK |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | In GenBank |
| Plaque Notes | clear center with turbid edges, diameter ~1-1.5 mm |
| Morphotype | Siphoviridae |
| Number of Genes | 61 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP978843 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
184 |
| Pitt Freezer Box Grid# |
E7 |
| Available Files |
| GenBank File for Phamerator | Download |