| Detailed Information for Phage PitaDog |
| Discovery Information |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Morgan Sperratore, Terri Smith and Karen Leach |
| Year Found | 2015 |
| Location Found | New Castle, DE USA |
| Finding Institution | Cabrini University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 21°C |
| GPS Coordinates | 39.67198 N, 75.635332 W Map |
| Discovery Notes | The soil was dry. |
| Naming Notes | PitaDog is the nickname for the family dog, Lucy. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 26, 2016 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 230 |
| Genome length (bp) | 42963 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 13 bases |
| Overhang Sequence | GGTAACCGTGATA |
| GC Content | 60.7% |
| Sequencing Notes | original sample mixed, 50% reads have 100% identity to Nightmare |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AK |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques are fairly large and grows better at RT with 22.5 mM calcium chloride. |
| Morphotype | Siphoviridae |
| Number of Genes | 61 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF140425 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
Cabrini College |
| SEA Lysate Titer |
1 X 10^7 pfu/ml |
| Date of SEA Lysate Titering |
Aug 1, 2015 |
| Pitt Freezer Box# |
149 |
| Pitt Freezer Box Grid# |
D1 |
| Available Files |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |