| Detailed Information for Phage Potatoes |
| Discovery Information |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Hannah Snell, Rachel Estrera |
| Year Found | 2017 |
| Location Found | Northampton, MA USA |
| Finding Institution | Smith College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.32 N, 72.636944 W Map |
| Discovery Notes | The weather was overcast, windy and the temperature was 23°C. The soil was dark brown, it had a couple of rocks and chips in it, but was otherwise of a fine consistency. It was approximately two inches below the top layer of wood-chips.
|
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 8, 2018 |
| Sequencing Facility | Smith College |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 43661 |
| Genome length (bp) | 43661 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 13 bases |
| Overhang Sequence | GGTAACCGTGATA |
| GC Content | 61.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AK |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | The petri dishes had multiple plaques for the targeted phage. A hazy appearance indicated a lysogenic phage, and clear plaques indicate a lytic phage. The plaques appeared to be clear, so it was denoted that the phages are lytic phages. |
| Morphotype | Siphoviridae |
| Number of Genes | 62 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK279901 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.33*10^8 phages/mL |
| Pitt Freezer Box# |
58 |
| Pitt Freezer Box Grid# |
H7 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
