Microbacterium phage Poultress
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Detailed Information for Phage Poultress
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByTanner Barwick
Year Found2023
Location FoundBrunswick, GA USA
Finding InstitutionCollege of Coastal Georgia
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature33°C
GPS Coordinates31.181628 N, 81.487338 W Map
Discovery NotesTaken from clipping of 1-2 day old grass.
Naming Notes“Poult”- for chicken and “ress”- for fortress.
Themed after chickens because of a more recent spot test where bacteria regrew in a cleared area. The bacteria regrowth in question looked like a sunny side up egg.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 11, 2024
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage1363
Genome length (bp)56564
Character of genome endsCircularly Permuted
GC Content63.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEF
Subcluster--
Cluster Life CycleLytic
Lysogeny NotesBacteria would sometimes regrow in areas cleared by phage. Occasional plaques exhibited bullseye-like morphology.
Other Cluster Members
Annotating InstitutionCollege of Coastal Georgia
Annotation StatusIn GenBank
Plaque NotesSmall, clear bullseye-like plaques. Plaques are approximately 1mm or smaller.
MorphotypeSiphoviridae
Number of Genes81
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Poultress_1

Poultress_2

Poultress_3

Poultress_4
(terminase small subunit)

Poultress_5

Poultress_6

Poultress_7

Poultress_8

Poultress_9

Poultress_10
(terminase large subunit)

Poultress_11

Poultress_12
(portal protein)

Poultress_13
(capsid maturation protease)

Poultress_14

Poultress_15
(scaffolding protein)

Poultress_16
(minor capsid protein)

Poultress_17
(major capsid protein)

Poultress_18

Poultress_19

Poultress_20
(head-to-tail stopper)

Poultress_21

Poultress_22
(tail terminator)

Poultress_23

Poultress_24

Poultress_25
(major tail protein)

Poultress_26
(tail assembly chaperone)

Poultress_27
(tail assembly chaperone)

Poultress_28
(tape measure protein)

Poultress_29
(minor tail protein)

Poultress_30
(minor tail protein)

Poultress_31
(minor tail protein)

Poultress_32
(minor tail protein)

Poultress_33

Poultress_34
(endolysin)

Poultress_35

Poultress_36

Poultress_37

Poultress_38

Poultress_39
(DNA helicase)

Poultress_40

Poultress_41

Poultress_42
(Cas4 exonuclease)

Poultress_43

Poultress_44
(ASCE ATPase)

Poultress_45

Poultress_46
(DNA primase/helicase)

Poultress_47

Poultress_48

Poultress_49

Poultress_50

Poultress_51
(oxidoreductase)

Poultress_52
(ThyX-like thymidylate synthase)

Poultress_53

Poultress_54

Poultress_55
(DnaE-like DNA polymerase III alpha)

Poultress_56
(DnaE-like DNA polymerase III alpha)

Poultress_57

Poultress_58

Poultress_59

Poultress_60

Poultress_61

Poultress_62

Poultress_63

Poultress_64

Poultress_65

Poultress_66

Poultress_67

Poultress_68

Poultress_69

Poultress_70

Poultress_71

Poultress_72
(RuvC-like resolvase)

Poultress_73

Poultress_74

Poultress_75

Poultress_76

Poultress_77

Poultress_78

Poultress_79

Poultress_80

Poultress_81

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPQ114744
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 5X10E10
Pitt Freezer Box# 183
Pitt Freezer Box Grid# G10
Available Files
GenBank File for PhameratorDownload