Streptomyces phage Stella
Stella EM Picture
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Detailed Information for Phage Stella
Discovery Information
Isolation HostStreptomyces viridochromogenes DSM40736
Found ByKylie Mink and Guanlan Dong
Year Found2015
Location FoundClayton, MO USA
Finding InstitutionWashington University in St. Louis
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature29°C
GPS Coordinates38.643893 N, 90.315244 W Map
Discovery NotesThe soil sample was taken from a patch of ivy underneath a small tree outside of a dorm building window. The soil was fairly dry and was found in an urban setting.
Naming NotesWe named the phage Stella due to the star-shaped plaques the phage formed.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJul 21, 2022
Sequencing FacilityThe McDonnell Genome Institute at Washington University
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage226
Genome length (bp)46511
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length263 bp
GC Content60.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBF
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionWashington University in St. Louis
Annotation StatusIn GenBank
Plaque NotesAfter five rounds of purification, we obtained a plate with plaques that were all the same medium size. The plaques were star-shaped with a round, clear center and cloudy, rough edges.
MorphotypePodoviridae
Number of Genes69
Number of tRNAs21
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Stella_1

Stella_2
(terminase)

Stella_3
(endolysin)

Stella_4
(portal protein)

Stella_5

Stella_6

Stella_7

Stella_8

Stella_9

Stella_10

Stella_11

Stella_12
(minor tail protein)

Stella_13
(minor tail protein)

Stella_14

Stella_15 — tRNA
(tRNA-Pro(tgg))

Stella_16 — tRNA
(tRNA-Gly(tcc))

Stella_17 — tRNA
(tRNA-Met(cat))

Stella_18 — tRNA
(tRNA-Trp(cca))

Stella_19 — tRNA
(tRNA-Gln(ctg))

Stella_20 — tRNA
(tRNA-Ser(aga))

Stella_21 — tRNA
(tRNA-Ser(gct))

Stella_22 — tRNA
(tRNA-Tyr(gta))

Stella_23 — tRNA
(tRNA-Cys(gca))

Stella_24 — tRNA
(tRNA-Ala(tgc))

Stella_25 — tRNA
(tRNA-Ile(gat))

Stella_26 — tRNA
(tRNA-Asp(gtc))

Stella_27 — tRNA
(tRNA-Leu(tag))

Stella_28 — tRNA
(tRNA-Phe(gaa))

Stella_29 — tRNA
(tRNA-His(gtg))

Stella_30 — tRNA
(tRNA-Arg(acg))

Stella_31 — tRNA
(tRNA-Lys(ctt))

Stella_32 — tRNA
(tRNA-Glu(ctc))

Stella_33 — tRNA
(tRNA-Thr(agt))

Stella_34 — tRNA
(tRNA-Asn(gtt))

Stella_35 — tRNA
(tRNA-Val(tac))

Stella_36

Stella_37
(major capsid protein)

Stella_38
(minor tail protein)

Stella_39

Stella_40
(minor tail protein)

Stella_41

Stella_42

Stella_43

Stella_44

Stella_45

Stella_46

Stella_47

Stella_48

Stella_49

Stella_50

Stella_51
(holin)

Stella_52

Stella_53

Stella_54

Stella_55

Stella_56

Stella_57

Stella_58

Stella_59

Stella_60

Stella_61
(DNA primase)

Stella_62
(DNA primase)

Stella_63
(holliday junction resolvase)

Stella_64
(DnaB-like dsDNA helicase)

Stella_65

Stella_66

Stella_67
(Cas4 family exonuclease)

Stella_68
(WhiB family transcription factor)

Stella_69
(helix-turn-helix DNA binding domain protein)

Stella_70
(metallophosphoesterase)

Stella_71
(helix-turn-helix DNA binding domain protein)

Stella_72

Stella_73

Stella_74

Stella_75

Stella_76

Stella_77

Stella_78

Stella_79
(ArdA-like antirestriction protein)

Stella_80
(helix-turn-helix DNA binding domain protein)

Stella_81

Stella_82

Stella_83

Stella_84

Stella_85

Stella_86

Stella_87

Stella_88

Stella_89

Stella_90

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOP751154
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Designator Stella
SEA Lysate Titer 2.1 x 10^8 pfu/mL
Date of SEA Lysate Titering Oct 13, 2015
Available Files
GenBank File for PhameratorDownload