| Detailed Information for Phage Stigma |
| Discovery Information |
| Isolation Host | Streptomyces griseus ATCC 10137 |
| Found By | Sydney Gans |
| Year Found | 2020 |
| Location Found | Denton, TX United States |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 33.1227 N, 97.919 W Map |
| Discovery Notes | Abigail Higginbotham Collected soil from (33.1227 N, 97.919 W) at 690 feet elevation in the south west side of the University of North Texas campus. It was 93 degrees at the time of collection. The soil was dry and dark, as it had retained water from rain a few days prior. It was collected under a tree by a parking lot. Abigail collected the top 1 inch of the soil and placed it in a plastic bag. I adopted one of the phages from her soil sample. |
| Naming Notes | I named this phage after a song I always play to fall asleep. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 22, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1246 |
| Genome length (bp) | 129455 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 734 bp |
| GC Content | 47.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BK |
| Subcluster | BK1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | Mostly round, some more oval shaped, bullseye plaques about 2mm or less |
| Number of Genes | 233 |
| Number of tRNAs | 33 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON456343 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.8 x 10^9 pfu/mL |
| Pitt Freezer Box# |
118 |
| Pitt Freezer Box Grid# |
A7 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
