| Detailed Information for Phage Timinator |
| Discovery Information |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Timothy Philip |
| Year Found | 2014 |
| Location Found | Frisco, TX USA |
| Finding Institution | Baylor University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 33.168693 N, 96.754697 W Map |
| Discovery Notes | The soil sample was from a construction site in close proximity to heavy duty construction, the road and a Porta-Potty. Furthermore, the soil sample was next to a lot of construction material, like wood, concrete powder and bricks. |
| Naming Notes | Earlier in the process, a lot of plates were completely lysed and the bacteria was completely terminated. Therefore, since my name is Timothy and my phage was terminating the bacteria, I thought Timinator would be a clever play on words. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 20, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 3520 |
| Genome length (bp) | 51138 |
| Character of genome ends | Unknown |
| GC Content | 60.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AO |
| Subcluster | AO2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques changed size over time as it started off as small plaques but during the purification process, the plaques grew in size. The average diameter of the plaques were approximately 3.5 mm. |
| Morphotype | Myoviridae |
| Number of Genes | 79 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF377441 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4*10^10 pfu/ml |
| Date of SEA Lysate Titering |
Dec 1, 2014 |
| Pitt Freezer Box# |
148 |
| Pitt Freezer Box Grid# |
F11 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
