| Detailed Information for Phage YoyoKar | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Gia Sohi, Yonal Karandana |
| Year Found | 2025 |
| Location Found | Las Vegas, NV United States |
| Finding Institution | University of Nevada Las Vegas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 25°C |
| GPS Coordinates | 36.243333 N, 115.325278 W Map |
| Discovery Notes | The sample was collected near the roots of a potted green chili plant, with a 31°C outside temperature, 18% humidity, and 11 mph wind. |
| Naming Notes | Named after the people who found the phage. "Yoyo" refers to Yonal and "Kar" refers to Gia. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 15, 2026 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 738 |
| Genome length (bp) | 70883 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 4 bases |
| Overhang Sequence | GTCT |
| GC Content | 65.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | O |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | University of Nevada Las Vegas |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | Turbid and cloudy plaques with faint halos around them. Average plaque size is 0.2 mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 123 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewYoyoKar_1 YoyoKar_2 YoyoKar_3 YoyoKar_4 YoyoKar_5 YoyoKar_6 YoyoKar_7 YoyoKar_8 YoyoKar_9 YoyoKar_10 YoyoKar_11 YoyoKar_12 YoyoKar_13 YoyoKar_14 YoyoKar_15 YoyoKar_16 YoyoKar_17 YoyoKar_18 YoyoKar_19 YoyoKar_20 YoyoKar_21 YoyoKar_22 YoyoKar_23 YoyoKar_24 YoyoKar_25 YoyoKar_26 YoyoKar_27 YoyoKar_28 YoyoKar_29 YoyoKar_30 YoyoKar_31 YoyoKar_32 YoyoKar_33 YoyoKar_34 YoyoKar_35 YoyoKar_36 YoyoKar_37 YoyoKar_38 YoyoKar_39 YoyoKar_40 YoyoKar_41 YoyoKar_42 YoyoKar_43 YoyoKar_44 YoyoKar_45 YoyoKar_46 YoyoKar_47 YoyoKar_48 YoyoKar_49 YoyoKar_50 YoyoKar_51 YoyoKar_52 YoyoKar_53 YoyoKar_54 YoyoKar_55 YoyoKar_56 YoyoKar_57 YoyoKar_58 YoyoKar_59 YoyoKar_60 YoyoKar_61 YoyoKar_62 YoyoKar_63 YoyoKar_64 YoyoKar_65 YoyoKar_66 YoyoKar_67 YoyoKar_68 YoyoKar_69 YoyoKar_70 YoyoKar_71 YoyoKar_72 YoyoKar_73 YoyoKar_74 YoyoKar_75 YoyoKar_76 YoyoKar_77 YoyoKar_78 YoyoKar_79 YoyoKar_80 YoyoKar_81 YoyoKar_82 YoyoKar_83 YoyoKar_84 YoyoKar_85 YoyoKar_86 YoyoKar_87 YoyoKar_88 YoyoKar_89 YoyoKar_90 YoyoKar_91 YoyoKar_92 YoyoKar_93 YoyoKar_94 YoyoKar_95 YoyoKar_96 YoyoKar_97 YoyoKar_98 YoyoKar_99 YoyoKar_100 YoyoKar_101 YoyoKar_102 YoyoKar_103 YoyoKar_104 YoyoKar_105 YoyoKar_106 YoyoKar_107 YoyoKar_108 YoyoKar_109 YoyoKar_110 YoyoKar_111 YoyoKar_112 YoyoKar_113 YoyoKar_114 YoyoKar_115 YoyoKar_116 YoyoKar_117 YoyoKar_118 YoyoKar_119 YoyoKar_120 YoyoKar_121 YoyoKar_122 YoyoKar_123 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 2.7 x 10^10 PFU/mL |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
