| Detailed Information for Phage Zakhe101 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Zakhe M Mcunu |
| Year Found | 2014 |
| Location Found | Durban, South Africa |
| Finding Institution | University of Kwazulu-Natal |
| Program | Mycobacterial Genetics Course, Durban, South Africa |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 29.817371 S, 30.942245 E Map |
| Discovery Notes | This phage was found near the laboratoy soil, the soil that it was extracted from was moist and dark. The colonies were so small and grow after a 24hr incubation at 37C. It is location 3A on the lysate prepared |
| Naming Notes | The name of this phage comes directly to my own name Zakhe meaning 'make'. This phage is morphologically different compare to other phages that were isolated as it had a prolate head and tail. the code 101 means that it usually have a spot that include plaques inside a rare feacture in the immunity test. Therefore its name is Zakhe101 |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 29, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 215 |
| Genome length (bp) | 69653 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 4 bases |
| Overhang Sequence | GTGT |
| GC Content | 65.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | O |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques were a circular shape. the head of the phage is prolate similar to corndog and Dylan phages. From the digest picture it can be seen against Da Vinci on position 2 that it forms a clear spot. |
| Morphotype | Siphoviridae |
| Number of Genes | 123 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KT281796 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
7 |
| Pitt Freezer Box Grid# |
F1 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
