| Detailed Information for Phage Zakhe101 | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Zakhe M Mcunu |
| Year Found | 2014 |
| Location Found | Durban, South Africa |
| Finding Institution | University of Kwazulu-Natal |
| Program | Mycobacterial Genetics Course, Durban, South Africa |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 29.817371 S, 30.942245 E Map |
| Discovery Notes | This phage was found near the laboratoy soil, the soil that it was extracted from was moist and dark. The colonies were so small and grow after a 24hr incubation at 37C. It is location 3A on the lysate prepared |
| Naming Notes | The name of this phage comes directly to my own name Zakhe meaning 'make'. This phage is morphologically different compare to other phages that were isolated as it had a prolate head and tail. the code 101 means that it usually have a spot that include plaques inside a rare feacture in the immunity test. Therefore its name is Zakhe101 |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 29, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 215 |
| Genome length (bp) | 69653 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 4 bases |
| Overhang Sequence | GTGT |
| GC Content | 65.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | O |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques were a circular shape. the head of the phage is prolate similar to corndog and Dylan phages. From the digest picture it can be seen against Da Vinci on position 2 that it forms a clear spot. |
| Morphotype | Siphoviridae |
| Number of Genes | 123 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewZakhe101_1 Zakhe101_2 Zakhe101_3 Zakhe101_4 Zakhe101_5 Zakhe101_6 Zakhe101_7 Zakhe101_8 Zakhe101_9 Zakhe101_10 Zakhe101_11 Zakhe101_12 Zakhe101_13 Zakhe101_14 Zakhe101_15 Zakhe101_16 Zakhe101_17 Zakhe101_18 Zakhe101_19 Zakhe101_20 Zakhe101_21 Zakhe101_22 Zakhe101_23 Zakhe101_24 Zakhe101_25 Zakhe101_26 Zakhe101_27 Zakhe101_28 Zakhe101_29 Zakhe101_30 Zakhe101_31 Zakhe101_32 Zakhe101_33 Zakhe101_34 Zakhe101_35 Zakhe101_36 Zakhe101_37 Zakhe101_38 Zakhe101_39 Zakhe101_40 Zakhe101_41 Zakhe101_42 Zakhe101_43 Zakhe101_44 Zakhe101_45 Zakhe101_46 Zakhe101_47 Zakhe101_48 Zakhe101_49 Zakhe101_50 Zakhe101_51 Zakhe101_52 Zakhe101_53 Zakhe101_54 Zakhe101_55 Zakhe101_56 Zakhe101_57 Zakhe101_58 Zakhe101_59 Zakhe101_60 Zakhe101_61 Zakhe101_62 Zakhe101_63 Zakhe101_64 Zakhe101_65 Zakhe101_66 Zakhe101_67 Zakhe101_68 Zakhe101_69 Zakhe101_70 Zakhe101_71 Zakhe101_72 Zakhe101_73 Zakhe101_74 Zakhe101_75 Zakhe101_76 Zakhe101_77 Zakhe101_78 Zakhe101_79 Zakhe101_80 Zakhe101_81 Zakhe101_82 Zakhe101_83 Zakhe101_84 Zakhe101_85 Zakhe101_86 Zakhe101_87 Zakhe101_88 Zakhe101_89 Zakhe101_90 Zakhe101_91 Zakhe101_92 Zakhe101_93 Zakhe101_94 Zakhe101_95 Zakhe101_96 Zakhe101_97 Zakhe101_98 Zakhe101_99 Zakhe101_100 Zakhe101_101 Zakhe101_102 Zakhe101_103 Zakhe101_104 Zakhe101_105 Zakhe101_106 Zakhe101_107 Zakhe101_108 Zakhe101_109 Zakhe101_110 Zakhe101_111 Zakhe101_112 Zakhe101_113 Zakhe101_114 Zakhe101_115 Zakhe101_116 Zakhe101_117 Zakhe101_118 Zakhe101_119 Zakhe101_120 Zakhe101_121 Zakhe101_122 Zakhe101_123 |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KT281796 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 7 |
| Pitt Freezer Box Grid# | F1 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
